U.S. Department of EnergyOffice of Scientific and Technical Information
divergence in basal transcriptomes of "Wild Type" Chlamydomonas reinhardtii strains
Abstract
This is a study to compare the basal transcriptomes of several widely used laboratory strains of the Chlorophyte alga, Chlamydomonas reinhardtii. Given that there is a high degree of genetic diversity among the closely-related laboratory strains, we wished to examine how much variation there is at the transcriptome level. A panel of WT strains (CC-124, CC-125, CC-1009, CC-1690, CC-1691), all believed to be descended from a single zygospore isolated in 1945, were chosen based on their representing the oldest lineages among the standard laboratory strains. Additionally, CC-4532, which was the source for the current (v6) reference assembly, and CC-4533, which is the initial parental strain of the CLiP library collection of mutant strains, were also included in this study based on their significance to the Chlamydomonas community. All strains were grown in liquid cultures under identical, mixotrophic conditions (light + acetate) to mid-log phase before collecting mRNA for RNA-Seq analysis. Overall design: Pre-cultures of each strain (CC-124, CC-125, CC-1009, CC-1690, CC-1691, CC-4532, and CC-4533) were used to innoculate flasks of Tris-Acetate-Phosphate (TAP) media supplemented with Kropat's trace metals at a concentration of 1.5 x 10^4 cells/mL. Flasks were placed on a shaking platform at 180 RPM under 50-70 µmol xmore »
- Publication Date:
- Other Number(s):
- GSE296941; PRJNA1261968
- DOE Contract Number:
- SC0020627
- Research Org.:
- The Regents of University of California
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)
- OSTI Identifier:
- 3004932
- DOI:
- https://doi.org/10.11578/3004932
Citation Formats
divergence in basal transcriptomes of "Wild Type" Chlamydomonas reinhardtii strains. United States: N. p., 2025.
Web. doi:10.11578/3004932.
divergence in basal transcriptomes of "Wild Type" Chlamydomonas reinhardtii strains. United States. doi:https://doi.org/10.11578/3004932
2025.
"divergence in basal transcriptomes of "Wild Type" Chlamydomonas reinhardtii strains". United States. doi:https://doi.org/10.11578/3004932. https://www.osti.gov/servlets/purl/3004932. Pub date:Tue Nov 04 23:00:00 EST 2025
@article{osti_3004932,
title = {divergence in basal transcriptomes of "Wild Type" Chlamydomonas reinhardtii strains},
abstractNote = {This is a study to compare the basal transcriptomes of several widely used laboratory strains of the Chlorophyte alga, Chlamydomonas reinhardtii. Given that there is a high degree of genetic diversity among the closely-related laboratory strains, we wished to examine how much variation there is at the transcriptome level. A panel of WT strains (CC-124, CC-125, CC-1009, CC-1690, CC-1691), all believed to be descended from a single zygospore isolated in 1945, were chosen based on their representing the oldest lineages among the standard laboratory strains. Additionally, CC-4532, which was the source for the current (v6) reference assembly, and CC-4533, which is the initial parental strain of the CLiP library collection of mutant strains, were also included in this study based on their significance to the Chlamydomonas community. All strains were grown in liquid cultures under identical, mixotrophic conditions (light + acetate) to mid-log phase before collecting mRNA for RNA-Seq analysis. Overall design: Pre-cultures of each strain (CC-124, CC-125, CC-1009, CC-1690, CC-1691, CC-4532, and CC-4533) were used to innoculate flasks of Tris-Acetate-Phosphate (TAP) media supplemented with Kropat's trace metals at a concentration of 1.5 x 10^4 cells/mL. Flasks were placed on a shaking platform at 180 RPM under 50-70 µmol x m^-2 x s^-1 of light. Cultures were grown until they reached ~2-3 x 10^6 cells/mL, about three days, before total RNA was collected and subjected RNA-Seq analysis.},
doi = {10.11578/3004932},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 04 23:00:00 EST 2025},
month = {Tue Nov 04 23:00:00 EST 2025}
}