U.S. Department of EnergyOffice of Scientific and Technical Information
16S rRNA squencing to identify microbiomes associated with different crops and different growing conditions
Abstract
The microbiome associated with plant roots, the rhizobiome, can harbor beneficial microbes that alleviate stress, but the factors influencing their recruitment are unclear. We conducted a greenhouse experiment using field soil with a legacy of growing switchgrass and common bean to investigate the impact of short-term drought severity on the recruitment of active bacterial rhizobiome members. We applied 16S rRNA and 16S rRNA gene sequencing for both crops and metabolite profiling for switchgrass. We included planted and unplanted conditions to distinguish environment- versus plant-mediated rhizobiome drivers.
- Authors:
-
- Laboratoire Ecologie Microbienne at the University of Lyon, France
- Publication Date:
- DOE Contract Number:
- SC0018409
- Research Org.:
- Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- Subject:
- 16S rRNA sequencing; microbiome; plants
- OSTI Identifier:
- 3003596
- DOI:
- https://doi.org/10.82250/3003596
Citation Formats
Shade, Ashley. 16S rRNA squencing to identify microbiomes associated with different crops and different growing conditions. United States: N. p., 2022.
Web. doi:10.82250/3003596.
Shade, Ashley. 16S rRNA squencing to identify microbiomes associated with different crops and different growing conditions. United States. doi:https://doi.org/10.82250/3003596
Shade, Ashley. 2022.
"16S rRNA squencing to identify microbiomes associated with different crops and different growing conditions". United States. doi:https://doi.org/10.82250/3003596. https://www.osti.gov/servlets/purl/3003596. Pub date:Wed Jul 27 04:00:00 UTC 2022
@article{osti_3003596,
title = {16S rRNA squencing to identify microbiomes associated with different crops and different growing conditions},
author = {Shade, Ashley},
abstractNote = {The microbiome associated with plant roots, the rhizobiome, can harbor beneficial microbes that alleviate stress, but the factors influencing their recruitment are unclear. We conducted a greenhouse experiment using field soil with a legacy of growing switchgrass and common bean to investigate the impact of short-term drought severity on the recruitment of active bacterial rhizobiome members. We applied 16S rRNA and 16S rRNA gene sequencing for both crops and metabolite profiling for switchgrass. We included planted and unplanted conditions to distinguish environment- versus plant-mediated rhizobiome drivers.},
doi = {10.82250/3003596},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jul 27 04:00:00 UTC 2022},
month = {Wed Jul 27 04:00:00 UTC 2022}
}
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