PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth in Saccharomyces cerevisiae

Gasch, Audrey

doi:10.82250/3003539

Title: PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth in Saccharomyces cerevisiae

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Abstract

Past work from our lab, focused on engineering the budding yeast Saccharomyces cerevisiae for fermentation of the non-native pentose sugar xylose, discovered that hyperactivation of the RAS/Protein Kinase A (PKA) pathway was needed for rapid anaerobic xylose fermentation. Interestingly, the mechanism of PKA hyperactivation has a dramatic impact on growth and metabolism on xylose; deletion of the RAS inhibitor IRA2 permits rapid growth and fermentation, while deletion of the PKA regulatory subunit BCY1 allows for fermentation without growth on xylose. To understand how a single deletion in the PKA pathway can decouple growth and metabolism, we performed transcriptomic analysis of these strains, predicting that altered PKA activity would impact global gene expression and identify pathways important for growth and metabolism coordination.

Authors:

Gasch, Audrey

GLBRC - University of Wisconsin-Madison

Publication Date:: Mon May 01 00:00:00 EDT 2023

DOE Contract Number:: SC0018409

Research Org.:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 3003539

DOI:: https://doi.org/10.82250/3003539

Citation Formats


                    Gasch, Audrey. PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth in Saccharomyces cerevisiae.  United States: N. p., 2023. 
        Web.  doi:10.82250/3003539.

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                    Gasch, Audrey. PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth in Saccharomyces cerevisiae.  United States.  doi:https://doi.org/10.82250/3003539

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                    Gasch, Audrey. 2023.  
"PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth in Saccharomyces cerevisiae".  United States.  doi:https://doi.org/10.82250/3003539.  https://www.osti.gov/servlets/purl/3003539. Pub date:Mon May 01 00:00:00 EDT 2023

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@article{osti_3003539,

  title        = {PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth in Saccharomyces cerevisiae},

  author       = {Gasch, Audrey},

  abstractNote = {Past work from our lab, focused on engineering the budding yeast Saccharomyces cerevisiae for fermentation of the non-native pentose sugar xylose, discovered that hyperactivation of the RAS/Protein Kinase A (PKA) pathway was needed for rapid anaerobic xylose fermentation. Interestingly, the mechanism of PKA hyperactivation has a dramatic impact on growth and metabolism on xylose; deletion of the RAS inhibitor IRA2 permits rapid growth and fermentation, while deletion of the PKA regulatory subunit BCY1 allows for fermentation without growth on xylose. To understand how a single deletion in the PKA pathway can decouple growth and metabolism, we performed transcriptomic analysis of these strains, predicting that altered PKA activity would impact global gene expression and identify pathways important for growth and metabolism coordination.},

  doi          = {10.82250/3003539},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Mon May 01 00:00:00 EDT 2023},

  month        = {Mon May 01 00:00:00 EDT 2023}

}

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DOI: https://doi.org/10.82250/3003539

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PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth in Saccharomyces cerevisiae

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