U.S. Department of EnergyOffice of Scientific and Technical Information
Natural variation in the consequences of gene overexpression during osmotic stress [BAR-Seq]
Abstract
We conducted a barcode sequencing experiment examining 4 yeast strains expressing a high copy number plasmid library. For each strain, we took samples at generation 0 (start of experiment) and generation 10 to quantify how plasmid abundance changes over time when strains were grown under osmotic and ionic stress induced by 0.7M of sodium chloride (NaCl). Each strain was measured in biological triplicate for a total of 24 samples.
- Authors:
-
- GLBRC - University of Wisconsin-Madison
- Publication Date:
- DOE Contract Number:
- SC0018409
- Research Org.:
- Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- Subject:
- GxE; copy-number variation; evolution; fitness cost
- OSTI Identifier:
- 3003537
- DOI:
- https://doi.org/10.82250/3003537
Citation Formats
Gasch, Audrey. Natural variation in the consequences of gene overexpression during osmotic stress [BAR-Seq]. United States: N. p., 2023.
Web. doi:10.82250/3003537.
Gasch, Audrey. Natural variation in the consequences of gene overexpression during osmotic stress [BAR-Seq]. United States. doi:https://doi.org/10.82250/3003537
Gasch, Audrey. 2023.
"Natural variation in the consequences of gene overexpression during osmotic stress [BAR-Seq]". United States. doi:https://doi.org/10.82250/3003537. https://www.osti.gov/servlets/purl/3003537. Pub date:Fri Jun 30 00:00:00 EDT 2023
@article{osti_3003537,
title = {Natural variation in the consequences of gene overexpression during osmotic stress [BAR-Seq]},
author = {Gasch, Audrey},
abstractNote = {We conducted a barcode sequencing experiment examining 4 yeast strains expressing a high copy number plasmid library. For each strain, we took samples at generation 0 (start of experiment) and generation 10 to quantify how plasmid abundance changes over time when strains were grown under osmotic and ionic stress induced by 0.7M of sodium chloride (NaCl). Each strain was measured in biological triplicate for a total of 24 samples.},
doi = {10.82250/3003537},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jun 30 00:00:00 EDT 2023},
month = {Fri Jun 30 00:00:00 EDT 2023}
}
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