U.S. Department of EnergyOffice of Scientific and Technical Information
Metagenome-assembled genomes from Wind River Basin floodplain sediments Riverton, Wyoming site (August 2015)
Abstract
Microorganisms play a key role in cycling nutrients and contaminants in the terrestrial environment depending on their genetic potential. Here we present metagenome-assembled genomes (MAGs) for the bacterial and archaeal community in floodplain sediment samples taken August 29, 2015 at a location (KB1) close to DOE Legacy Management well 855 at the Riverton, Wyoming floodplain site in the Wind River Basin (WRB). The groundwater at this site exhibits persistent U, Mo, and sulfate plumes and is one of the field sites in focus for the SLAC Groundwater Quality SFA program. Sediment samples from a deep soil pit were collected from 0 to 234 cm depth below surface at discrete depths every ~10-20 cm for microbial analyses. 13 metagenomes were sequenced through JGI and can be found under Gold sequencing project: Gs0131241. Metagenomes were assembled, binned, and refined using metawrap to generate MAGs (>50% complete and < 10% contamination based on checkM scores).This dataset includes a zip file of 2216 MAG fasta files and a csv file with quality, taxonomic classification (GTDB RS220), and metagenome accessions for MAGs. This dataset also includes a file-level metadata (flmd.csv) file that lists each file contained in the dataset with associated metadata and a datamore »
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- Stanford Linear Accelerator Center - National Accelerator Laboratory
- Stanford University
- Publication Date:
- DOE Contract Number:
- AC02-05CH11231
- Research Org.:
- Groundwater Quality SFA
- Sponsoring Org.:
- U.S. DOE > Office of Science > Biological and Environmental Research (BER)
- Subject:
- 54 ENVIRONMENTAL SCIENCES; EARTH SCIENCE > BIOSPHERE > OMICS > BUILDING BLOCKS > CONTIG:GCMD; EARTH SCIENCE > BIOSPHERE > OMICS > BUILDING BLOCKS > eDNA:GCMD; EARTH SCIENCE > BIOSPHERE > OMICS > METAGENOME > METAGENOME ASSEMBLED GENOMES (MAGs):GCMD; EARTH SCIENCE > BIOSPHERE > OMICS > MICROBIOME:GCMD; EARTH SCIENCE > BIOSPHERE > OMICS > OMICS METHODOLOGY > BIN/BINNING:GCMD; EARTH SCIENCE > LAND SURFACE > SOILS; EARTH SCIENCE > LAND SURFACE > SOILS > MICROFAUNA; ESS-DIVE CSV File Formatting Guidelines Reporting Format; ESS-DIVE File Level Metadata Reporting Format; related identifiers
- OSTI Identifier:
- 2563565
- DOI:
- https://doi.org/10.15485/2563565
Citation Formats
Rasmussen, Anna, Langenfeld, Kathryn, Cardarelli, Emily, Maher, Kate, Bargar, John, Boye, Kristin, and Francis, Chris. Metagenome-assembled genomes from Wind River Basin floodplain sediments Riverton, Wyoming site (August 2015). United States: N. p., 2024.
Web. doi:10.15485/2563565.
Rasmussen, Anna, Langenfeld, Kathryn, Cardarelli, Emily, Maher, Kate, Bargar, John, Boye, Kristin, & Francis, Chris. Metagenome-assembled genomes from Wind River Basin floodplain sediments Riverton, Wyoming site (August 2015). United States. doi:https://doi.org/10.15485/2563565
Rasmussen, Anna, Langenfeld, Kathryn, Cardarelli, Emily, Maher, Kate, Bargar, John, Boye, Kristin, and Francis, Chris. 2024.
"Metagenome-assembled genomes from Wind River Basin floodplain sediments Riverton, Wyoming site (August 2015)". United States. doi:https://doi.org/10.15485/2563565. https://www.osti.gov/servlets/purl/2563565. Pub date:Tue Dec 31 23:00:00 EST 2024
@article{osti_2563565,
title = {Metagenome-assembled genomes from Wind River Basin floodplain sediments Riverton, Wyoming site (August 2015)},
author = {Rasmussen, Anna and Langenfeld, Kathryn and Cardarelli, Emily and Maher, Kate and Bargar, John and Boye, Kristin and Francis, Chris},
abstractNote = {Microorganisms play a key role in cycling nutrients and contaminants in the terrestrial environment depending on their genetic potential. Here we present metagenome-assembled genomes (MAGs) for the bacterial and archaeal community in floodplain sediment samples taken August 29, 2015 at a location (KB1) close to DOE Legacy Management well 855 at the Riverton, Wyoming floodplain site in the Wind River Basin (WRB). The groundwater at this site exhibits persistent U, Mo, and sulfate plumes and is one of the field sites in focus for the SLAC Groundwater Quality SFA program. Sediment samples from a deep soil pit were collected from 0 to 234 cm depth below surface at discrete depths every ~10-20 cm for microbial analyses. 13 metagenomes were sequenced through JGI and can be found under Gold sequencing project: Gs0131241. Metagenomes were assembled, binned, and refined using metawrap to generate MAGs (>50% complete and < 10% contamination based on checkM scores).This dataset includes a zip file of 2216 MAG fasta files and a csv file with quality, taxonomic classification (GTDB RS220), and metagenome accessions for MAGs. This dataset also includes a file-level metadata (flmd.csv) file that lists each file contained in the dataset with associated metadata and a data dictionary (dd.csv) file that contains column/row headers used throughout the files along with a definition, units, and data type},
doi = {10.15485/2563565},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 31 23:00:00 EST 2024},
month = {Tue Dec 31 23:00:00 EST 2024}
}