U.S. Department of EnergyOffice of Scientific and Technical Information
Mechanisms of regulation of the rhizosphere, roots and shoots of naive poplars
Abstract
Trees are associated with a broad range of microorganisms colonising the diverse tissues of their host. However, the early dynamics of the microbiota assembly microbiota from the root to shoot axis and how it is linked to root exudates and metabolite contents of tissues remain unclear. Here, we characterised how fungal and bacterial communities are altering root exudates as well as root and shoot metabolomes in parallel with their establishment in poplar cuttings (Populus tremula x tremuloides clone T89) over 30 days of growth. Sterile poplar cuttings were planted in natural or gamma irradiated soils. Bulk and rhizospheric soils, root and shoot tissues were collected from day 1 to day 30 to track the dynamic changes of fungal and bacterial communities in the different habitats by DNA metabarcoding. Root exudates and root and shoot metabolites were analysed in parallel by gas chromatography-mass spectrometry.
- Authors:
-
- French National Research Institute for Agriculture, Food and Environment (INRAE), Champenoux (France)
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States) Plant Microbe Interfaces
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States) Plant-Microbe Interfaces
- Publication Date:
- DOE Contract Number:
- AC05-00OR22725
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- Subject:
- 09 BIOMASS FUELS; PMI
- OSTI Identifier:
- 2556848
- DOI:
- https://doi.org/10.25983/2556848
Citation Formats
Deveau, Aurelie, Frachia, F, Engle, Nancy L, and Tschaplinski, Timothy J. Mechanisms of regulation of the rhizosphere, roots and shoots of naive poplars. United States: N. p., 2024.
Web. doi:10.25983/2556848.
Deveau, Aurelie, Frachia, F, Engle, Nancy L, & Tschaplinski, Timothy J. Mechanisms of regulation of the rhizosphere, roots and shoots of naive poplars. United States. doi:https://doi.org/10.25983/2556848
Deveau, Aurelie, Frachia, F, Engle, Nancy L, and Tschaplinski, Timothy J. 2024.
"Mechanisms of regulation of the rhizosphere, roots and shoots of naive poplars". United States. doi:https://doi.org/10.25983/2556848. https://www.osti.gov/servlets/purl/2556848. Pub date:Sun Dec 08 19:00:00 EST 2024
@article{osti_2556848,
title = {Mechanisms of regulation of the rhizosphere, roots and shoots of naive poplars},
author = {Deveau, Aurelie and Frachia, F and Engle, Nancy L and Tschaplinski, Timothy J},
abstractNote = {Trees are associated with a broad range of microorganisms colonising the diverse tissues of their host. However, the early dynamics of the microbiota assembly microbiota from the root to shoot axis and how it is linked to root exudates and metabolite contents of tissues remain unclear. Here, we characterised how fungal and bacterial communities are altering root exudates as well as root and shoot metabolomes in parallel with their establishment in poplar cuttings (Populus tremula x tremuloides clone T89) over 30 days of growth. Sterile poplar cuttings were planted in natural or gamma irradiated soils. Bulk and rhizospheric soils, root and shoot tissues were collected from day 1 to day 30 to track the dynamic changes of fungal and bacterial communities in the different habitats by DNA metabarcoding. Root exudates and root and shoot metabolites were analysed in parallel by gas chromatography-mass spectrometry.},
doi = {10.25983/2556848},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Dec 08 19:00:00 EST 2024},
month = {Sun Dec 08 19:00:00 EST 2024}
}