U.S. Department of EnergyOffice of Scientific and Technical Information
EVT 16s Data and Large Supplementary Files
Abstract
Soil microorganisms often interact to carry out decomposition of complex organic carbon and nitrogen compounds, such as chitin, but the high diversity and complexity of the soil microbiome and habitat has posed a challenge to elucidating such interactions between soil microorganisms. Here, we seek to address this challenge through analysis of a model soil consortium (MSC-2) of eight soil bacterial species. Our aim was to elucidate specific roles of the member species during chitin metabolism. Samples were collected from MSC-2 incubated in chitin-enriched soil over three months. Multi-omics was used to understand how the community composition, transcripts, proteins and chitin decomposition shifted over time. The data clearly and consistently revealed a temporal shift during chitin decomposition with defined contributions by individual species. A Streptomyces genus member (sp001905665) was a key player in early steps of chitin decomposition, with other MSC-2 members being central in carrying out later steps. These results illustrate how multi-omics applied to a defined consortium untangles interactions between soil microorganisms.
- Authors:
-
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Pacific Northwest National Laboratory
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Publication Date:
- Other Number(s):
- PNNL-SA-209438
- DOE Contract Number:
- AC05-76RL01830
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- NASA
- OSTI Identifier:
- 2530740
- DOI:
- https://doi.org/10.25584/2530740
Citation Formats
McClure, Ryan, and Jansson, Janet. EVT 16s Data and Large Supplementary Files. United States: N. p., 2024.
Web. doi:10.25584/2530740.
McClure, Ryan, & Jansson, Janet. EVT 16s Data and Large Supplementary Files. United States. doi:https://doi.org/10.25584/2530740
McClure, Ryan, and Jansson, Janet. 2024.
"EVT 16s Data and Large Supplementary Files". United States. doi:https://doi.org/10.25584/2530740. https://www.osti.gov/servlets/purl/2530740. Pub date:Tue Dec 31 23:00:00 EST 2024
@article{osti_2530740,
title = {EVT 16s Data and Large Supplementary Files},
author = {McClure, Ryan and Jansson, Janet},
abstractNote = {Soil microorganisms often interact to carry out decomposition of complex organic carbon and nitrogen compounds, such as chitin, but the high diversity and complexity of the soil microbiome and habitat has posed a challenge to elucidating such interactions between soil microorganisms. Here, we seek to address this challenge through analysis of a model soil consortium (MSC-2) of eight soil bacterial species. Our aim was to elucidate specific roles of the member species during chitin metabolism. Samples were collected from MSC-2 incubated in chitin-enriched soil over three months. Multi-omics was used to understand how the community composition, transcripts, proteins and chitin decomposition shifted over time. The data clearly and consistently revealed a temporal shift during chitin decomposition with defined contributions by individual species. A Streptomyces genus member (sp001905665) was a key player in early steps of chitin decomposition, with other MSC-2 members being central in carrying out later steps. These results illustrate how multi-omics applied to a defined consortium untangles interactions between soil microorganisms.},
doi = {10.25584/2530740},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 31 23:00:00 EST 2024},
month = {Tue Dec 31 23:00:00 EST 2024}
}