U.S. Department of EnergyOffice of Scientific and Technical Information
Next-generation sequencing dataset of genome-scale CRISPRi in Synechococcus sp. PCC 7002 across seven conditions
Abstract
A 33,298-member sgRNA library developed for Synechococus sp. PCC 7002 was screened with two replicates across seven growth conditions and sequenced with Illumina NextSeq (paired end, 2x150 bp) for a total of ~700M reads. The original plasmid library and the library after transformation into a dCas9-containing and dCas9-absent strain were also sequenced as a reference for initial sgRNA abundance.
- Authors:
-
- Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO, 80304, USA
- Biosciences Division Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA
- Department of Biochemistry, University of Colorado, Boulder, CO, 80309, USA Renewable and Sustainable Energy Institute, University of Colorado, Boulder, CO, 80309, USA National Renewable Energy Laboratory, Golden, CO, 80401, USA
- Publication Date:
- DOE Contract Number:
- SC0018368
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Earth & Environmental Systems Science (EESS)
- Subject:
- genome- wide screens environmental acclimation spectral tuning photosynthesis energy transduction
- OSTI Identifier:
- 2525933
- DOI:
- https://doi.org/10.25983/2525933
Citation Formats
Hren, Andrew, Lollini, Nicole, Carper, Dana L, Abraham, Paul, Cameron, Jeffery C, Fox, Jerome M, and Eckert, Carrie. Next-generation sequencing dataset of genome-scale CRISPRi in Synechococcus sp. PCC 7002 across seven conditions. United States: N. p., 2025.
Web. doi:10.25983/2525933.
Hren, Andrew, Lollini, Nicole, Carper, Dana L, Abraham, Paul, Cameron, Jeffery C, Fox, Jerome M, & Eckert, Carrie. Next-generation sequencing dataset of genome-scale CRISPRi in Synechococcus sp. PCC 7002 across seven conditions. United States. doi:https://doi.org/10.25983/2525933
Hren, Andrew, Lollini, Nicole, Carper, Dana L, Abraham, Paul, Cameron, Jeffery C, Fox, Jerome M, and Eckert, Carrie. 2025.
"Next-generation sequencing dataset of genome-scale CRISPRi in Synechococcus sp. PCC 7002 across seven conditions". United States. doi:https://doi.org/10.25983/2525933. https://www.osti.gov/servlets/purl/2525933. Pub date:Mon Mar 03 04:00:00 UTC 2025
@article{osti_2525933,
title = {Next-generation sequencing dataset of genome-scale CRISPRi in Synechococcus sp. PCC 7002 across seven conditions},
author = {Hren, Andrew and Lollini, Nicole and Carper, Dana L and Abraham, Paul and Cameron, Jeffery C and Fox, Jerome M and Eckert, Carrie},
abstractNote = {A 33,298-member sgRNA library developed for Synechococus sp. PCC 7002 was screened with two replicates across seven growth conditions and sequenced with Illumina NextSeq (paired end, 2x150 bp) for a total of ~700M reads. The original plasmid library and the library after transformation into a dCas9-containing and dCas9-absent strain were also sequenced as a reference for initial sgRNA abundance.},
doi = {10.25983/2525933},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Mar 03 04:00:00 UTC 2025},
month = {Mon Mar 03 04:00:00 UTC 2025}
}
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