U.S. Department of EnergyOffice of Scientific and Technical Information
RNA-Seq analysis of nine tRNA-Modification deficient mutants in E. coli
Abstract
We performed an RNA-Seq analysis of nine of E.coli single knockout strains from the Keio collection carrying deletions in tRNA modification genes (dusB, truA, truD, thiI, miaA, rlmN, mnmA, mnmC, mnmG) and wildtype. RNA was isolated from each of the different strains at grown in LB at 37˚C up to mid-exponential phase (OD(A600nm) ~0.6) in three biological replicates.
- Authors:
-
;
;
- Microbiology and Cell Science Dept, University of Florida, Gainesville FL 32611 Genetics Institute, University of Florida, Gainesville, FL 32611; DOE Systems Biology Knowledgebase
- Microbiology and Cell Science Dept, University of Florida, Gainesville FL 32611
- Antimicrobial Resistance Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, Singapore Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139
- Publication Date:
- Research Org.:
- Microbiology and Cell Science Dept, University of Florida, Gainesville FL 32611 Genetics Institute, University of Florida, Gainesville, FL 32611 Antimicrobial Resistance Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, Singapore Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- Subject:
- tRNA modification, transcriptomics, Escherichia coli
- OSTI Identifier:
- 2475943
- DOI:
- https://doi.org/10.25982/197146.10/2475943
Citation Formats
de Crécy-Lagard, Valérie, Babor, Jill, Bacusmo, Jo Marie, and Dedon, Peter C. RNA-Seq analysis of nine tRNA-Modification deficient mutants in E. coli. United States: N. p., 2024.
Web. doi:10.25982/197146.10/2475943.
de Crécy-Lagard, Valérie, Babor, Jill, Bacusmo, Jo Marie, & Dedon, Peter C. RNA-Seq analysis of nine tRNA-Modification deficient mutants in E. coli. United States. doi:https://doi.org/10.25982/197146.10/2475943
de Crécy-Lagard, Valérie, Babor, Jill, Bacusmo, Jo Marie, and Dedon, Peter C. 2024.
"RNA-Seq analysis of nine tRNA-Modification deficient mutants in E. coli". United States. doi:https://doi.org/10.25982/197146.10/2475943. https://www.osti.gov/servlets/purl/2475943. Pub date:Tue Nov 05 04:00:00 UTC 2024
@article{osti_2475943,
title = {RNA-Seq analysis of nine tRNA-Modification deficient mutants in E. coli},
author = {de Crécy-Lagard, Valérie and Babor, Jill and Bacusmo, Jo Marie and Dedon, Peter C.},
abstractNote = {We performed an RNA-Seq analysis of nine of E.coli single knockout strains from the Keio collection carrying deletions in tRNA modification genes (dusB, truA, truD, thiI, miaA, rlmN, mnmA, mnmC, mnmG) and wildtype. RNA was isolated from each of the different strains at grown in LB at 37˚C up to mid-exponential phase (OD(A600nm) ~0.6) in three biological replicates.},
doi = {10.25982/197146.10/2475943},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 05 04:00:00 UTC 2024},
month = {Tue Nov 05 04:00:00 UTC 2024}
}
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