Title: Lignin content of Populus trichocarpa residues after CELF pretreatment and CBP fermentation

Abstract

Here we present a dataset of lignin content from a woody energy crop (Populus trichocarpa) residues after a series of co-solvent enhanced lignocellulosic fractionation (CELF) pretreatment and consolidated bioprocessing (CBP) process. The natural poplar variant GW-9947 from the Center for Bioenergy Innovation (CBI) was used. The poplar was knife milled and passed through a 1 mm sieve and CELF pretreatment was performed in a Parr autoclave reactor with 7.5 wt % solids loading, 0.5 wt% H2SO4 as catalyst at 150°C with 5, 15, 25 and 30 minutes, respectively. Tetrahydrofuran was added in a 1:1 mass ratio with water as the pretreatment solvent. The residues from CELF pretreatment were then subjected to CBP using the bacterium C. thermocellum DSM 1313. CBP fermentations were performed at 60 °C in a shaker at 50, 75, and 100 grams/L solids loadings. The Klason lignin was measured using a two-step acid hydrolysis process. In brief, the poplar samples were first hydrolyzed by 72 wt% sulfuric acid at 30 oC for an hour. The hydrolysates were then diluted to 4 wt% sulfuric acid using deionized water and subsequently autoclaved at 121 oC for 1 h. Upon the completion of the two-step hydrolysis, the resulting solution wasmore » cooled to room temperature and the precipitate was then filtered through a G8 glass fiber filter through a crucible, dried at oven for overnight, and weighed to get the Klason lignin content. The lignin data provides information about lignin content changes after CELF and C. thermocellum CBP process.« less

Authors:

Pu, Yunqiao; Singh, Priyanka; Holwerda, Evert; Cai, Charles; Wyman, Charles; Ragauskas, Art

---

+ Show Author Affiliations

1. Joint Institute for Biological Sciences, Bioscience Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA
2. Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, Riverside, CA, USA
3. Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, Thayer School of Engineering, Dartmouth College, Hanover, NH, USA
4. Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, USA Department of Chemical and Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN, USA

Publication Date:

Wed Sep 18 04:00:00 UTC 2024

DOE Contract Number:  

AC05-00OR22725

Research Org.:

Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:

USDOE Office of Science (SC), Biological and Environmental Research (BER)

Subject:

Lignin Populus trichocarpa CELF pretreatment CBP fermentation, CBI

OSTI Identifier:

2446778

DOI:

https://doi.org/10.25983/2446778