U.S. Department of EnergyOffice of Scientific and Technical Information
Luteolibacter sp. strain Populi
Abstract
Luteolibacter sp. strain Populi is bacterium from the phylum Verrucomicrobiota, isolated from the rhizosphere of a black cottonwood tree, Populus trichocarpa, from the Cascade mountains in Washington. Its 6.6 Mb chromosome was completely sequenced using Oxford Nanopore long-reads and is predicted to encode 5301 proteins and 60 RNAs. The bacteria was isolated from the rhizosphere of a mature Populus trichocarpa from the Tieton riverwatershed of Washington state, USA (Lat: 46°42’9” N, Lon: 120°25 39’36” W). A rhizosphere sample (fine roots and adhering soil) was used to obtain a microbial fraction by centrifugation on Histodenz (12) and stained with 5µM Syto59 (Thermo Fisher Scientific Inc). A Cytopeia Influx cell sorter (BD, Franklin Lakes, NJ) was used to sort and array single cells (100 per plate) based on forward-side scatter and fluorescence intensity on asparagine-glucose nutrient agar (ATCC medium 184). The Luteolibacter sp. Populi genome sequence has been deposited in GenBank under the accession number CP161812. A draft genome annotated with Prokka and DRAM is available in this Narrative as Luteolibacter_sp_Prokka.240711.
- Authors:
-
;
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); DOE Systems Biology Knowledgebase
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- DOE Contract Number:
- AC05-00OR22725
- Research Org.:
- U.S. Department of Energy Systems Biology Knowledgebase
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; rhizosphere, plant-microbe interface
- OSTI Identifier:
- 2404572
- DOI:
- https://doi.org/10.25982/185119.92/2404572
Citation Formats
Allen, Benjamin, and Podar, Mircea. Luteolibacter sp. strain Populi. United States: N. p., 2024.
Web. doi:10.25982/185119.92/2404572.
Allen, Benjamin, & Podar, Mircea. Luteolibacter sp. strain Populi. United States. doi:https://doi.org/10.25982/185119.92/2404572
Allen, Benjamin, and Podar, Mircea. 2024.
"Luteolibacter sp. strain Populi". United States. doi:https://doi.org/10.25982/185119.92/2404572. https://www.osti.gov/servlets/purl/2404572. Pub date:Fri Jul 19 00:00:00 EDT 2024
@article{osti_2404572,
title = {Luteolibacter sp. strain Populi},
author = {Allen, Benjamin and Podar, Mircea},
abstractNote = {Luteolibacter sp. strain Populi is bacterium from the phylum Verrucomicrobiota, isolated from the rhizosphere of a black cottonwood tree, Populus trichocarpa, from the Cascade mountains in Washington. Its 6.6 Mb chromosome was completely sequenced using Oxford Nanopore long-reads and is predicted to encode 5301 proteins and 60 RNAs. The bacteria was isolated from the rhizosphere of a mature Populus trichocarpa from the Tieton riverwatershed of Washington state, USA (Lat: 46°42’9” N, Lon: 120°25 39’36” W). A rhizosphere sample (fine roots and adhering soil) was used to obtain a microbial fraction by centrifugation on Histodenz (12) and stained with 5µM Syto59 (Thermo Fisher Scientific Inc). A Cytopeia Influx cell sorter (BD, Franklin Lakes, NJ) was used to sort and array single cells (100 per plate) based on forward-side scatter and fluorescence intensity on asparagine-glucose nutrient agar (ATCC medium 184). The Luteolibacter sp. Populi genome sequence has been deposited in GenBank under the accession number CP161812. A draft genome annotated with Prokka and DRAM is available in this Narrative as Luteolibacter_sp_Prokka.240711.},
doi = {10.25982/185119.92/2404572},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jul 19 00:00:00 EDT 2024},
month = {Fri Jul 19 00:00:00 EDT 2024}
}