U.S. Department of EnergyOffice of Scientific and Technical Information
Seedling nitrogen uptake and rhizodeposition between mycorrhizal types
Abstract
Tree mycorrhizal associations are associated with patterns in N cycling and soil organic matter (SOM) storage, however, we still lack a mechanistic understanding of what tree and fungal traits drive these patterns and how they will respond to global changes in soil N availability. To address this knowledge gap, we investigated how arbuscular mycorrhizal (AM)- and ectomycorrhizal (EcM)-associated seedlings alter rhizodeposition in response to increased inorganic N acquisition. Specifically, we conducted this greenhouse experiment in a sealed labeling chamber with an enriched 13Carbon atmosphere and 15Nitrogen enriched fertilizer over the course of five months from April 2021 - August 2021. To include the variability across tree species, we grew eight species of seedlings belonging to eight families that were either arbuscular (Acer rubrum, Nyssa sylvatica, Thuja occidentalis, and Prunus seritina) or ectomycorrhizal-associated (Quercus rubra, Tilia americana, Pinus strobus, and Betula lenta). We measured rhizodeposition (mg 13C), plant N uptake from fertilizer (mg N), net soil carbon, and the abundance of mycorrhizal fungi (ITS sequencing and qPCR). We also characterized fungal (ITS2) and bacterial (16S) soil communities.The data from this project are ".csv" files that can up downloaded into a folder, and then run in the associated R markdown scripts aftermore »
- Authors:
-
;
- Oregon State University; ESS-DIVE
- Dartmouth College
- University of Wisconsin, Madison
- University of Georgia
- Publication Date:
- DOE Contract Number:
- SC0020228
- Research Org.:
- Environmental System Science Data Infrastructure for a Virtual Ecosystem; Testing mechanisms of how mycorrhizal associations affect forest soil carbon and nitrogen cycling
- Sponsoring Org.:
- U.S. DOE > Office of Science > Biological and Environmental Research (BER)
- Subject:
- 54 ENVIRONMENTAL SCIENCES; EARTH SCIENCE > AGRICULTURE > SOILS > NITROGEN; EARTH SCIENCE > ATMOSPHERE > AEROSOLS > NITRATE PARTICLES; EARTH SCIENCE > ATMOSPHERE > ATMOSPHERIC CHEMISTRY > NITROGEN COMPOUNDS > AMMONIA; EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > FUNGI; EARTH SCIENCE > BIOSPHERE > VEGETATION > BIOMASS; EARTH SCIENCE > LAND SURFACE > SOILS; EARTH SCIENCE > LAND SURFACE > SOILS > ORGANIC MATTER; EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ORGANIC CARBON
- OSTI Identifier:
- 2336869
- DOI:
- https://doi.org/10.15485/2336869
Citation Formats
Fitch, Amelia, Goldsmith, Sarah, Lankau, Richard, Wurzburger, Nina, Shortt, Zachary, Vrattos, Augustos, Laurent, Ella, and Hicks Pries, Caitlin. Seedling nitrogen uptake and rhizodeposition between mycorrhizal types. United States: N. p., 2024.
Web. doi:10.15485/2336869.
Fitch, Amelia, Goldsmith, Sarah, Lankau, Richard, Wurzburger, Nina, Shortt, Zachary, Vrattos, Augustos, Laurent, Ella, & Hicks Pries, Caitlin. Seedling nitrogen uptake and rhizodeposition between mycorrhizal types. United States. doi:https://doi.org/10.15485/2336869
Fitch, Amelia, Goldsmith, Sarah, Lankau, Richard, Wurzburger, Nina, Shortt, Zachary, Vrattos, Augustos, Laurent, Ella, and Hicks Pries, Caitlin. 2024.
"Seedling nitrogen uptake and rhizodeposition between mycorrhizal types". United States. doi:https://doi.org/10.15485/2336869. https://www.osti.gov/servlets/purl/2336869. Pub date:Mon Jan 01 04:00:00 UTC 2024
@article{osti_2336869,
title = {Seedling nitrogen uptake and rhizodeposition between mycorrhizal types},
author = {Fitch, Amelia and Goldsmith, Sarah and Lankau, Richard and Wurzburger, Nina and Shortt, Zachary and Vrattos, Augustos and Laurent, Ella and Hicks Pries, Caitlin},
abstractNote = {Tree mycorrhizal associations are associated with patterns in N cycling and soil organic matter (SOM) storage, however, we still lack a mechanistic understanding of what tree and fungal traits drive these patterns and how they will respond to global changes in soil N availability. To address this knowledge gap, we investigated how arbuscular mycorrhizal (AM)- and ectomycorrhizal (EcM)-associated seedlings alter rhizodeposition in response to increased inorganic N acquisition. Specifically, we conducted this greenhouse experiment in a sealed labeling chamber with an enriched 13Carbon atmosphere and 15Nitrogen enriched fertilizer over the course of five months from April 2021 - August 2021. To include the variability across tree species, we grew eight species of seedlings belonging to eight families that were either arbuscular (Acer rubrum, Nyssa sylvatica, Thuja occidentalis, and Prunus seritina) or ectomycorrhizal-associated (Quercus rubra, Tilia americana, Pinus strobus, and Betula lenta). We measured rhizodeposition (mg 13C), plant N uptake from fertilizer (mg N), net soil carbon, and the abundance of mycorrhizal fungi (ITS sequencing and qPCR). We also characterized fungal (ITS2) and bacterial (16S) soil communities.The data from this project are ".csv" files that can up downloaded into a folder, and then run in the associated R markdown scripts after changing the source folder location at the top of the script. These data include raw outputs and processed files (using the R markdown files) for seedling growth and biomass, 15N content, soil 13C content, raw reads and processed file versions for fungal and bacterial ASVS, and a final summary file used for modeling. R software is needed to run these data, and the packages needed are listed at the top of the R markdown file.},
doi = {10.15485/2336869},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 04:00:00 UTC 2024},
month = {Mon Jan 01 04:00:00 UTC 2024}
}