Data for "In vitro and in vivo NIR Fluorescence Lifetime Imaging with a time-gated SPAD camera"

Smith, Jason T.; Rudkouskaya, Alena; Gao, Shan; Gupta, Juhi M.; Ulku, Arin; Bruschini, Claudio; Charbon, Edoardo; Weiss, Shimon; Barroso, Margarida; Intes, Xavier; Michalet, Xavier

doi:10.6084/m9.figshare.16755514.v1

Title: Data for "In vitro and in vivo NIR Fluorescence Lifetime Imaging with a time-gated SPAD camera"

Abstract

Near-infrared (NIR) fluorescence lifetime imaging (FLI) provides a unique contrast mechanism to monitor biological parameters and molecular events in vivo. Single-photon avalanche diode (SPAD) cameras have been recently demonstrated in FLI microscopy (FLIM) applications, but their suitability for in vivo macroscopic FLI (MFLI) in deep tissues remains to be demonstrated. Herein, we report in vivo NIR MFLI measurement with SwissSPAD2, a large time-gated SPAD camera. We first benchmark its performance in well-controlled in vitro experiments, ranging from monitoring environmental effects on fluorescence lifetime, to quantifying Förster resonant energy transfer (FRET) between dyes. Next, we use it for in vivo studies of target-drug engagement in live and intact tumor xenografts using FRET. Information obtained with SwissSPAD2 was successfully compared to that obtained with a gated intensified charge-coupled device (ICCD) camera, using two different approaches. Our results demonstrate that SPAD cameras offer a powerful technology for in vivo preclinical applications in the NIR window.

Authors:

Smith, Jason T.; Rudkouskaya, Alena; Gao, Shan; Gupta, Juhi M.; Ulku, Arin; Bruschini, Claudio; Charbon, Edoardo; Weiss, Shimon; Barroso, Margarida; Intes, Xavier; Michalet, Xavier

Rensselaer Polytechnic Institute, Troy, NY (United States)
Albany Medical College, Albany, NY (United States)
Ecole Polytechnique Federale Lausanne (EPFL) (Switzerland)
University of California, Los Angeles, CA (United States)

Publication Date:: Wed Jan 05 00:00:00 EST 2022

DOE Contract Number:: SC0020338

Research Org.:: Univ. of California, Los Angeles, CA (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Subject:: 47 OTHER INSTRUMENTATION; 60 APPLIED LIFE SCIENCES

Keywords:: FRET; HER2; EGFR; Trastuzumab; Cetuximab; 2DG; Fluorescence lifetime imaging; ICCD; CMOS SPAD Array; time-gated detection; phasor; NLSF; in vivo molecular imaging; Biological Techniques; Cancer; Biomedical Instrumentation; Photodetectors, Optical Sensors and Solar Cells

OSTI Identifier:: 2217646

DOI:: https://doi.org/10.6084/m9.figshare.16755514.v1

Citation Formats


                    Smith, Jason T., Rudkouskaya, Alena, Gao, Shan, Gupta, Juhi M., Ulku, Arin, Bruschini, Claudio, Charbon, Edoardo, Weiss, Shimon, Barroso, Margarida, Intes, Xavier, and Michalet, Xavier. Data for "In vitro and in vivo NIR Fluorescence Lifetime Imaging with a time-gated SPAD camera".  United States: N. p., 2022. 
        Web.  doi:10.6084/m9.figshare.16755514.v1.

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                    Smith, Jason T., Rudkouskaya, Alena, Gao, Shan, Gupta, Juhi M., Ulku, Arin, Bruschini, Claudio, Charbon, Edoardo, Weiss, Shimon, Barroso, Margarida, Intes, Xavier, & Michalet, Xavier. Data for "In vitro and in vivo NIR Fluorescence Lifetime Imaging with a time-gated SPAD camera".  United States.  doi:https://doi.org/10.6084/m9.figshare.16755514.v1

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                    Smith, Jason T., Rudkouskaya, Alena, Gao, Shan, Gupta, Juhi M., Ulku, Arin, Bruschini, Claudio, Charbon, Edoardo, Weiss, Shimon, Barroso, Margarida, Intes, Xavier, and Michalet, Xavier. 2022.  
"Data for "In vitro and in vivo NIR Fluorescence Lifetime Imaging with a time-gated SPAD camera"".  United States.  doi:https://doi.org/10.6084/m9.figshare.16755514.v1.  https://www.osti.gov/servlets/purl/2217646. Pub date:Wed Jan 05 00:00:00 EST 2022

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@article{osti_2217646,

  title        = {Data for "In vitro and in vivo NIR Fluorescence Lifetime Imaging with a time-gated SPAD camera"},

  author       = {Smith, Jason T. and Rudkouskaya, Alena and Gao, Shan and Gupta, Juhi M. and Ulku, Arin and Bruschini, Claudio and Charbon, Edoardo and Weiss, Shimon and Barroso, Margarida and Intes, Xavier and Michalet, Xavier},

  abstractNote = {Near-infrared (NIR) fluorescence lifetime imaging (FLI) provides a unique contrast mechanism to monitor biological parameters and molecular events in vivo. Single-photon avalanche diode (SPAD) cameras have been recently demonstrated in FLI microscopy (FLIM) applications, but their suitability for in vivo macroscopic FLI (MFLI) in deep tissues remains to be demonstrated. Herein, we report in vivo NIR MFLI measurement with SwissSPAD2, a large time-gated SPAD camera. We first benchmark its performance in well-controlled in vitro experiments, ranging from monitoring environmental effects on fluorescence lifetime, to quantifying Förster resonant energy transfer (FRET) between dyes. Next, we use it for in vivo studies of target-drug engagement in live and intact tumor xenografts using FRET. Information obtained with SwissSPAD2 was successfully compared to that obtained with a gated intensified charge-coupled device (ICCD) camera, using two different approaches. Our results demonstrate that SPAD cameras offer a powerful technology for in vivo preclinical applications in the NIR window.},

  doi          = {10.6084/m9.figshare.16755514.v1},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Wed Jan 05 00:00:00 EST 2022},

  month        = {Wed Jan 05 00:00:00 EST 2022}

}

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DOI: https://doi.org/10.6084/m9.figshare.16755514.v1

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Works referencing / citing this record:

In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera
journal, May 2022

Smith, Jason T.; Rudkouskaya, Alena; Gao, Shan
Optica, Vol. 9, Issue 5
https://doi.org/10.1364/OPTICA.454790

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Title: Data for "In vitro and in vivo NIR Fluorescence Lifetime Imaging with a time-gated SPAD camera"

Abstract

Citation Formats

In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera journal, May 2022

In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera
journal, May 2022