Chloroform Fumigation Extraction for Microbial Biomass and Dissolved Organic Carbon from SPRUCE, Marcell Experimental Forest, Minnesota, 2021, 2022, and 2024

Buell, Zachary W,; Phillips, Jana R.; Birkebak, Joshua M.; Calkins, Skylar E.; Mead, Thomas J.; Ottinger, Sara L.; Lowe, Kenneth; Schadt, Christopher W.; Mayes, Melanie A .

doi:10.25581/spruce.109/1998876

Title: Chloroform Fumigation Extraction for Microbial Biomass and Dissolved Organic Carbon from SPRUCE, Marcell Experimental Forest, Minnesota, 2021, 2022, and 2024

Dataset
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Abstract

This data set provides the results for chloroform fumigation extraction (CFE) of peat samples collected from ambient and experimental plots in the Spruce and Peatland Responses Under Environmental Change (SPRUCE) Experiment site in June and August of 2021, June of 2022, and June, August, and October of 2024. The SPRUCE Experiment site is in the Marcell Experimental Forest in northern Minnesota, USA. The data set includes values for microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), dissolved organic carbon (DOC), dissolved nitrogen (DN), moisture content (MC, available for 2021 and 2022 only) and gravimetric water content (GWC) at 11 depth increments of two-meter peat cores taken from 12 sampling sites at SPRUCE (10 temperature treatment enclosures, 2 ambient temperature treatment enclosures). The sample analysis followed standard methods. The samples were analyzed using a Shimadzu Total Organic Carbon/Nitrogen (TOC/N) analyzer (TOC-V and TOC-L; 2021-2022) or an Elementar vario TOC Cube (2024), liquid catalytic oxidation combustion analyzers for total carbon and nitrogen analysis. This dataset contains two data files in comma separate (.csv) format. Additional metadata are provided: two data dictionaries and a file-level metadata file in comma separate (.csv) format and a user guide in PDF (*.pdf) format.

Authors:

; Phillips, Jana R.;

;

University of Minnesota, Saint Paul, MN (United States)
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Publication Date:: Sun Dec 31 23:00:00 EST 2023

DOE Contract Number:: AC05-00OR22725

Research Org.:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Subject:: Marcell Experimental Forest; Peatland; SPRUCE Experiment; Spruce and Peatland Responses Under Changing Environments; dissolved nitrogen; dissolved organic carbon; microbial biomass carbon; microbial biomass nitrogen

OSTI Identifier:: 1998876

DOI:: https://doi.org/10.25581/spruce.109/1998876

Citation Formats


                    Buell, Zachary W,, Phillips, Jana R., Birkebak, Joshua M., Calkins, Skylar E., Mead, Thomas J., Ottinger, Sara L., Lowe, Kenneth, Schadt, Christopher W., and Mayes, Melanie A . Chloroform Fumigation Extraction for Microbial Biomass and Dissolved Organic Carbon from SPRUCE, Marcell Experimental Forest, Minnesota, 2021, 2022, and 2024.  United States: N. p., 2023. 
        Web.  doi:10.25581/spruce.109/1998876.

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                    Buell, Zachary W,, Phillips, Jana R., Birkebak, Joshua M., Calkins, Skylar E., Mead, Thomas J., Ottinger, Sara L., Lowe, Kenneth, Schadt, Christopher W., & Mayes, Melanie A . Chloroform Fumigation Extraction for Microbial Biomass and Dissolved Organic Carbon from SPRUCE, Marcell Experimental Forest, Minnesota, 2021, 2022, and 2024.  United States.  doi:https://doi.org/10.25581/spruce.109/1998876

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                    Buell, Zachary W,, Phillips, Jana R., Birkebak, Joshua M., Calkins, Skylar E., Mead, Thomas J., Ottinger, Sara L., Lowe, Kenneth, Schadt, Christopher W., and Mayes, Melanie A . 2023.  
"Chloroform Fumigation Extraction for Microbial Biomass and Dissolved Organic Carbon from SPRUCE, Marcell Experimental Forest, Minnesota, 2021, 2022, and 2024".  United States.  doi:https://doi.org/10.25581/spruce.109/1998876.  https://www.osti.gov/servlets/purl/1998876. Pub date:Sun Dec 31 23:00:00 EST 2023

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@article{osti_1998876,

  title        = {Chloroform Fumigation Extraction for Microbial Biomass and Dissolved Organic Carbon from SPRUCE, Marcell Experimental Forest, Minnesota, 2021, 2022, and 2024},

  author       = {Buell, Zachary W, and Phillips, Jana R. and Birkebak, Joshua M. and Calkins, Skylar E. and Mead, Thomas J. and Ottinger, Sara L. and Lowe, Kenneth and Schadt, Christopher W. and Mayes, Melanie A .},

  abstractNote = {This data set provides the results for chloroform fumigation extraction (CFE) of peat samples collected from ambient and experimental plots in the Spruce and Peatland Responses Under Environmental Change (SPRUCE) Experiment site in June and August of 2021, June of 2022, and June, August, and October of 2024. The SPRUCE Experiment site is in the Marcell Experimental Forest in northern Minnesota, USA. The data set includes values for microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), dissolved organic carbon (DOC), dissolved nitrogen (DN), moisture content (MC, available for 2021 and 2022 only) and gravimetric water content (GWC) at 11 depth increments of two-meter peat cores taken from 12 sampling sites at SPRUCE (10 temperature treatment enclosures, 2 ambient temperature treatment enclosures). The sample analysis followed standard methods. The samples were analyzed using a Shimadzu Total Organic Carbon/Nitrogen (TOC/N) analyzer (TOC-V and TOC-L; 2021-2022) or an Elementar vario TOC Cube (2024), liquid catalytic oxidation combustion analyzers for total carbon and nitrogen analysis. This dataset contains two data files in comma separate (.csv) format. Additional metadata are provided: two data dictionaries and a file-level metadata file in comma separate (.csv) format and a user guide in PDF (*.pdf) format.},

  doi          = {10.25581/spruce.109/1998876},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Sun Dec 31 23:00:00 EST 2023},

  month        = {Sun Dec 31 23:00:00 EST 2023}

}

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DOI: https://doi.org/10.25581/spruce.109/1998876

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