Kinetic and temperature sensitivity properties of soil exoenzymes through the soil profile down to one-meter depth at a temperate coniferous forest (Blodgett, CA)

Alves, Ricardo J Eloy; Callejas, Ileana A; Marschmann, Gianna L; Mooshammer, Maria; Singh, Hans W; Whitney, Bizuayehu; Torn, Margaret S; Brodie, Eoin L

doi:10.15485/1830417

Title: Kinetic and temperature sensitivity properties of soil exoenzymes through the soil profile down to one-meter depth at a temperate coniferous forest (Blodgett, CA)

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Abstract

This dataset contains data on kinetic and temperature sensitivity parameters of the exoenzymes β-glucosidase (BG), leucine/leucyl aminopeptidase (LAP) and acid phosphatase (AP) across a soil profile down to 90 cm depth at Blodgett forest, CA, USA. These data were generated to determine if kinetic and thermal properties of microbial exoenzymes involved in organic matter decomposition varied with soil depth, following variation in soil properties and microbial communities, as part of the study: Alves et al. (2021). Kinetic Properties of Microbial Exoenzymes Vary with Soil Depth but Have Similar Temperature Sensitivities Through the Soil Profile. Frontiers in Microbiology 12:3618. https://doi.org/10.3389/fmicb.2021.735282. This research was performed within the framework of the TES Belowground Biogeochemistry SFA project, in particular association with a long-term field warming experiment of the whole soil profile at Blodgett forest. Samples for this work were collected from locations representative of the field experimental plots.Potential enzyme activity rates were measured using laboratory fluorometric assays with soils collected at 0-10, 10-20, 30-40, 50-60, 60-70 and 80-90 cm deep in biological triplicates (i.e., three soil cores collected at different representative locations). Assays with each soil were conducted over a gradient of eight substrate concentrations per enzyme, and incubated at 4, 10, 16, 25,more »« less

Authors:

; Callejas, Ileana A;

;

; Singh, Hans W; Whitney, Bizuayehu;

;

Lawrence Berkeley National Laboratory; Lawrence Berkeley National Laboratory
Lawrence Berkeley National Laboratory, University of California Los Angeles
Lawrence Berkeley National Laboratory
University of California Berkeley
Lawrence Berkeley National Laboratory, University of California Berkeley

Publication Date:: Thu Dec 31 23:00:00 EST 2020

Research Org.:: Environmental System Science Data Infrastructure for a Virtual Ecosystem; Terrestrial Ecosystem Science at Berkeley Lab

Sponsoring Org.:: US.DOE. > Office of Science > Biological and Environmental Research (BER)

Subject:: 54 ENVIRONMENTAL SCIENCES; ARRHENIUS; EARTH SCIENCE > LAND SURFACE > SOILS; EARTH SCIENCE > LAND SURFACE > SOILS > CARBON; EARTH SCIENCE > LAND SURFACE > SOILS > ENZYME ACTIVATION ENERGY EA; EARTH SCIENCE > LAND SURFACE > SOILS > ENZYME ACTIVITY RATE; EARTH SCIENCE > LAND SURFACE > SOILS > ENZYME CATALYTIC EFFICIENCY; EARTH SCIENCE > LAND SURFACE > SOILS > ENZYME CHANGE IN HEAT CAPACITY ΔCp‡; EARTH SCIENCE > LAND SURFACE > SOILS > ENZYME HALF-SATURATION CONSTANT KM; EARTH SCIENCE > LAND SURFACE > SOILS > ENZYME KINETICS; EARTH SCIENCE > LAND SURFACE > SOILS > ENZYME MAXIMUM VELOCITY VMAX; EARTH SCIENCE > LAND SURFACE > SOILS > ENZYME POINT OF MAXIMUM TEMPERATURE SENSITIVITY TSMAX; EARTH SCIENCE > LAND SURFACE > SOILS > ENZYME Q10; EARTH SCIENCE > LAND SURFACE > SOILS > ENZYME TEMPERATURE COEFFICIENT Q10; EARTH SCIENCE > LAND SURFACE > SOILS > ENZYME TEMPERATURE OPTIMUM TOPT; EARTH SCIENCE > LAND SURFACE > SOILS > ENZYME TEMPERATURE SENSITIVITY; EARTH SCIENCE > LAND SURFACE > SOILS > EXTRACELLULAR ENZYMES; EARTH SCIENCE > LAND SURFACE > SOILS > MICROBIAL BIOMASS CARBON; EARTH SCIENCE > LAND SURFACE > SOILS > MICROBIAL BIOMASS NITROGEN; EARTH SCIENCE > LAND SURFACE > SOILS > MICROBIAL TRAITS; EARTH SCIENCE > LAND SURFACE > SOILS > NITROGEN; EARTH SCIENCE > LAND SURFACE > SOILS > ORGANIC MATTER; EARTH SCIENCE > LAND SURFACE > SOILS > SOIL DEPTH; EARTH SCIENCE > LAND SURFACE > SOILS > SOIL MOISTURE/WATER CONTENT; EARTH SCIENCE > LAND SURFACE > SOILS > SUBSOILS; ESS-DIVE CSV File Formatting Guidelines Reporting Format; ESS-DIVE File Level Metadata Reporting Format; ESS-DIVE Sample ID and Metadata Reporting Format; MACROMOLECULAR RATE THEORY; MICHAELIS-MENTEN

OSTI Identifier:: 1830417

DOI:: https://doi.org/10.15485/1830417

Citation Formats


                    Alves, Ricardo J Eloy, Callejas, Ileana A, Marschmann, Gianna L, Mooshammer, Maria, Singh, Hans W, Whitney, Bizuayehu, Torn, Margaret S, and Brodie, Eoin L. Kinetic and temperature sensitivity properties of soil exoenzymes through the soil profile down to one-meter depth at a temperate coniferous forest (Blodgett, CA).  United States: N. p., 2020. 
        Web.  doi:10.15485/1830417.

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                    Alves, Ricardo J Eloy, Callejas, Ileana A, Marschmann, Gianna L, Mooshammer, Maria, Singh, Hans W, Whitney, Bizuayehu, Torn, Margaret S, & Brodie, Eoin L. Kinetic and temperature sensitivity properties of soil exoenzymes through the soil profile down to one-meter depth at a temperate coniferous forest (Blodgett, CA).  United States.  doi:https://doi.org/10.15485/1830417

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                    Alves, Ricardo J Eloy, Callejas, Ileana A, Marschmann, Gianna L, Mooshammer, Maria, Singh, Hans W, Whitney, Bizuayehu, Torn, Margaret S, and Brodie, Eoin L. 2020.  
"Kinetic and temperature sensitivity properties of soil exoenzymes through the soil profile down to one-meter depth at a temperate coniferous forest (Blodgett, CA)".  United States.  doi:https://doi.org/10.15485/1830417.  https://www.osti.gov/servlets/purl/1830417. Pub date:Thu Dec 31 23:00:00 EST 2020

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@article{osti_1830417,

  title        = {Kinetic and temperature sensitivity properties of soil exoenzymes through the soil profile down to one-meter depth at a temperate coniferous forest (Blodgett, CA)},

  author       = {Alves, Ricardo J Eloy and Callejas, Ileana A and Marschmann, Gianna L and Mooshammer, Maria and Singh, Hans W and Whitney, Bizuayehu and Torn, Margaret S and Brodie, Eoin L},

  abstractNote = {This dataset contains data on kinetic and temperature sensitivity parameters of the exoenzymes β-glucosidase (BG), leucine/leucyl aminopeptidase (LAP) and acid phosphatase (AP) across a soil profile down to 90 cm depth at Blodgett forest, CA, USA. These data were generated to determine if kinetic and thermal properties of microbial exoenzymes involved in organic matter decomposition varied with soil depth, following variation in soil properties and microbial communities, as part of the study: Alves et al. (2021). Kinetic Properties of Microbial Exoenzymes Vary with Soil Depth but Have Similar Temperature Sensitivities Through the Soil Profile. Frontiers in Microbiology 12:3618. https://doi.org/10.3389/fmicb.2021.735282. This research was performed within the framework of the TES Belowground Biogeochemistry SFA project, in particular association with a long-term field warming experiment of the whole soil profile at Blodgett forest. Samples for this work were collected from locations representative of the field experimental plots.Potential enzyme activity rates were measured using laboratory fluorometric assays with soils collected at 0-10, 10-20, 30-40, 50-60, 60-70 and 80-90 cm deep in biological triplicates (i.e., three soil cores collected at different representative locations). Assays with each soil were conducted over a gradient of eight substrate concentrations per enzyme, and incubated at 4, 10, 16, 25, 35 or 50°C. Enzyme Michaelis-Menten kinetics were modeled over the eight substrate concentrations at each temperature, and the temperature sensitivity of the kinetic parameters was modeled over the six temperatures using linear Arrhenius/Q10 and non-linear Macromolecular Rate Theory (MMRT) models.The dataset includes the fully processed enzyme activity rate data used to model Michaelis-Menten kinetics, calculated kinetic and temperature sensitivity parameters, and basic soil and microbial biomass chemistry for each sample. All data is provided for each individual biological replicate, and kinetic and temperature sensitivity parameters are also provided as means of the biological replicates. The dataset also includes all raw measurement data and code used to parse, combine and perform the analyses described by Alves et al. (2021). For file descriptions, see the file-level metadata files: “enzymes_dataProcessed_flmd.xlsx” (processed data); “enzymes_dataRaw_flmd.xlsx” (compressed raw data and metadata); and “enzymes_code_flmd.xlsx” (compressed code). The experimental design, list of parameters measured, soil and microbial biomass chemistry data, and means of biological replicates for kinetic and temperature sensitivity parameters are also provided as human-readable tables in file “enzymeTraits_design_results_tables.xlsx”.},

  doi          = {10.15485/1830417},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Thu Dec 31 23:00:00 EST 2020},

  month        = {Thu Dec 31 23:00:00 EST 2020}

}

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DOI: https://doi.org/10.15485/1830417

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