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Title: Structural insights into the mechanism of rhodopsin phosphodiesterase

Abstract

Crystal structures of TMD of Rhodopsin phosphodiesterase (Rh-PDE). 6160 small-wedge (10°/crystal) datasets collected from loop-harvested microcrystals using EIGER X 9M detector at a wavelength of 1 Å on BL32XU, SPring-8. Beam size was around 15×10 µm2 and oscillation step was 0.1°. The crystals belonged to space group P212121 with unit cell parameters a~66, b~74, c~117 Å. 864 datasets were indexed and integrated using XDS with KAMO pipeline. Finally 271 integrated results were merged at 2.6 Å resolution after CC-based clustering and outlier rejection by KAMO in the published result.

Authors:
Publication Date:
Other Number(s):
CXIDB ID 171
DOE Contract Number:  
AC02-05CH11231
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Coherent X-ray Imaging Data Bank; University of Tokyo
Sponsoring Org.:
University of Tokyo
Keywords:
XFEL; BL32XU; X-ray Free-electorn Lasers; SPring-8; RhPDE-TMD; Synchrotron Crystallography (small-wedge)
OSTI Identifier:
1679953
DOI:
https://doi.org/10.11577/1679953

Citation Formats

Ikuta, Tatsuya. Structural insights into the mechanism of rhodopsin phosphodiesterase. United States: N. p., 2020. Web. doi:10.11577/1679953.
Ikuta, Tatsuya. Structural insights into the mechanism of rhodopsin phosphodiesterase. United States. doi:https://doi.org/10.11577/1679953
Ikuta, Tatsuya. 2020. "Structural insights into the mechanism of rhodopsin phosphodiesterase". United States. doi:https://doi.org/10.11577/1679953. https://www.osti.gov/servlets/purl/1679953. Pub date:Wed Oct 21 00:00:00 EDT 2020
@article{osti_1679953,
title = {Structural insights into the mechanism of rhodopsin phosphodiesterase},
author = {Ikuta, Tatsuya},
abstractNote = {Crystal structures of TMD of Rhodopsin phosphodiesterase (Rh-PDE). 6160 small-wedge (10°/crystal) datasets collected from loop-harvested microcrystals using EIGER X 9M detector at a wavelength of 1 Å on BL32XU, SPring-8. Beam size was around 15×10 µm2 and oscillation step was 0.1°. The crystals belonged to space group P212121 with unit cell parameters a~66, b~74, c~117 Å. 864 datasets were indexed and integrated using XDS with KAMO pipeline. Finally 271 integrated results were merged at 2.6 Å resolution after CC-based clustering and outlier rejection by KAMO in the published result.},
doi = {10.11577/1679953},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {10}
}