Structural insights into the mechanism of rhodopsin phosphodiesterase

Ikuta, Tatsuya

doi:10.11577/1679953

Title: Structural insights into the mechanism of rhodopsin phosphodiesterase

Abstract

Crystal structures of TMD of Rhodopsin phosphodiesterase (Rh-PDE). 6160 small-wedge (10°/crystal) datasets collected from loop-harvested microcrystals using EIGER X 9M detector at a wavelength of 1 Å on BL32XU, SPring-8. Beam size was around 15×10 µm2 and oscillation step was 0.1°. The crystals belonged to space group P212121 with unit cell parameters a~66, b~74, c~117 Å. 864 datasets were indexed and integrated using XDS with KAMO pipeline. Finally 271 integrated results were merged at 2.6 Å resolution after CC-based clustering and outlier rejection by KAMO in the published result.

Authors:: Ikuta, Tatsuya

Publication Date:: Wed Oct 21 00:00:00 EDT 2020

Other Number(s):: CXIDB ID 171

DOE Contract Number:: AC02-05CH11231

Research Org.:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Coherent X-ray Imaging Data Bank; University of Tokyo

Sponsoring Org.:: University of Tokyo

Keywords:: XFEL; BL32XU; X-ray Free-electorn Lasers; SPring-8; RhPDE-TMD; Synchrotron Crystallography (small-wedge)

OSTI Identifier:: 1679953

DOI:: https://doi.org/10.11577/1679953

Citation Formats


                    Ikuta, Tatsuya. Structural insights into the mechanism of rhodopsin phosphodiesterase.  United States: N. p., 2020. 
        Web.  doi:10.11577/1679953.

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                    Ikuta, Tatsuya. Structural insights into the mechanism of rhodopsin phosphodiesterase.  United States.  doi:https://doi.org/10.11577/1679953

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                    Ikuta, Tatsuya. 2020.  
"Structural insights into the mechanism of rhodopsin phosphodiesterase".  United States.  doi:https://doi.org/10.11577/1679953.  https://www.osti.gov/servlets/purl/1679953. Pub date:Wed Oct 21 00:00:00 EDT 2020

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@article{osti_1679953,

  title        = {Structural insights into the mechanism of rhodopsin phosphodiesterase},

  author       = {Ikuta, Tatsuya},

  abstractNote = {Crystal structures of TMD of Rhodopsin phosphodiesterase (Rh-PDE). 6160 small-wedge (10°/crystal) datasets collected from loop-harvested microcrystals using EIGER X 9M detector at a wavelength of 1 Å on BL32XU, SPring-8. Beam size was around 15×10 µm2 and oscillation step was 0.1°. The crystals belonged to space group P212121 with unit cell parameters a~66, b~74, c~117 Å. 864 datasets were indexed and integrated using XDS with KAMO pipeline. Finally 271 integrated results were merged at 2.6 Å resolution after CC-based clustering and outlier rejection by KAMO in the published result.},

  doi          = {10.11577/1679953},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Wed Oct 21 00:00:00 EDT 2020},

  month        = {Wed Oct 21 00:00:00 EDT 2020}

}

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DOI: https://doi.org/10.11577/1679953

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