SPRUCE High-Resolution Minirhizotrons in an Experimentally-Warmed Peatland Provide an Unprecedented Glimpse at Fine Roots and their Fungal Partners: Supporting Data

Childs, J.; Defrenne, C.E.; Brice, D.J.; Woodward, J.; Holbrook, K.N.; Nettles, W.R.; Taggart, M.; Iversen, C.M.

doi:10.25581/spruce.081/1637336

Title: SPRUCE High-Resolution Minirhizotrons in an Experimentally-Warmed Peatland Provide an Unprecedented Glimpse at Fine Roots and their Fungal Partners: Supporting Data

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Abstract

Images were collected using first of their kind, non-destructive, high-resolution automated minirhizotrons (RhizoSystems, LLC) to assess the response of plant fine-root and fungal mycelium dynamics to elevated temperatures after 4-6 years of whole-ecosystem warming and exposure to elevated carbon dioxide concentrations (e[CO2]) in a peat bog where the SPRUCE experiment is located. We focused on two SPRUCE experimental plots: Plot 10 has elevated temperature (+9°C) and plot 19 is a control (+0°C). Both have elevated CO2 (e[CO2]). Changes in root and fungal abundance with warming were estimated from a timeseries of landscape-level mosaiced images for each plot by measuring the proportional abundance of five belowground classes: fine roots of vascular plants, ectomycorrhizas, fungal hyphae, fungal rhizomorphs, and fungal sporocarps. To examine root and fungal phenology responses to warming, the length per individual root or fungal structure areal coverage were measured per image area of a set of timeseries patch-level mosaiced images for each plot. The experimental work was conducted in a Picea mariana [black spruce] – Sphagnum spp. bog forest in northern Minnesota, 40 km north of Grand Rapids, in the USDA Forest Service Marcell Experimental Forest (MEF). This ecosystem, which is located at the southern margin of the borealmore »« less

Authors:

Childs, J.; Defrenne, C.E.; Brice, D.J.; Woodward, J.; Holbrook, K.N.;

; Taggart, M.;

Publication Date:: Wed Jan 01 00:00:00 EST 2020

DOE Contract Number:: AC05-00OR22725

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Earth and Environmental Systems Science Division

Subject:: 54 ENVIRONMENTAL SCIENCES

Keywords:: plant fine-root, fungal mycelium, automated minirhizotrons, abundance, phenology, ectomycorrhizas, fungal hyphae, fungal rhizomorphs, fungal sporocarps, image analyses

Geolocation:: 47.50656,-93.45256|47.5047,-93.45256|47.5047,-93.45399|47.50656,-93.45399|47.50656,-93.45256

OSTI Identifier:: 1637336

DOI:: https://doi.org/10.25581/spruce.081/1637336

Project Location:

Citation Formats


                    Childs, J., Defrenne, C.E., Brice, D.J., Woodward, J., Holbrook, K.N., Nettles, W.R., Taggart, M., and Iversen, C.M. SPRUCE High-Resolution Minirhizotrons in an Experimentally-Warmed Peatland Provide an Unprecedented Glimpse at Fine Roots and their Fungal Partners: Supporting Data.  United States: N. p., 2020. 
        Web.  doi:10.25581/spruce.081/1637336.

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                    Childs, J., Defrenne, C.E., Brice, D.J., Woodward, J., Holbrook, K.N., Nettles, W.R., Taggart, M., & Iversen, C.M. SPRUCE High-Resolution Minirhizotrons in an Experimentally-Warmed Peatland Provide an Unprecedented Glimpse at Fine Roots and their Fungal Partners: Supporting Data.  United States.  doi:https://doi.org/10.25581/spruce.081/1637336

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                    Childs, J., Defrenne, C.E., Brice, D.J., Woodward, J., Holbrook, K.N., Nettles, W.R., Taggart, M., and Iversen, C.M. 2020.  
"SPRUCE High-Resolution Minirhizotrons in an Experimentally-Warmed Peatland Provide an Unprecedented Glimpse at Fine Roots and their Fungal Partners: Supporting Data".  United States.  doi:https://doi.org/10.25581/spruce.081/1637336.  https://www.osti.gov/servlets/purl/1637336. Pub date:Wed Jan 01 00:00:00 EST 2020

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@article{osti_1637336,

  title        = {SPRUCE High-Resolution Minirhizotrons in an Experimentally-Warmed Peatland Provide an Unprecedented Glimpse at Fine Roots and their Fungal Partners: Supporting Data},

  author       = {Childs, J. and Defrenne, C.E. and Brice, D.J. and Woodward, J. and Holbrook, K.N. and Nettles, W.R. and Taggart, M. and Iversen, C.M.},

  abstractNote = {Images were collected using first of their kind, non-destructive, high-resolution automated minirhizotrons (RhizoSystems, LLC) to assess the response of plant fine-root and fungal mycelium dynamics to elevated temperatures after 4-6 years of whole-ecosystem warming and exposure to elevated carbon dioxide concentrations (e[CO2]) in a peat bog where the SPRUCE experiment is located. We focused on two SPRUCE experimental plots: Plot 10 has elevated temperature (+9°C) and plot 19 is a control (+0°C). Both have elevated CO2 (e[CO2]). Changes in root and fungal abundance with warming were estimated from a timeseries of landscape-level mosaiced images for each plot by measuring the proportional abundance of five belowground classes: fine roots of vascular plants, ectomycorrhizas, fungal hyphae, fungal rhizomorphs, and fungal sporocarps. To examine root and fungal phenology responses to warming, the length per individual root or fungal structure areal coverage were measured per image area of a set of timeseries patch-level mosaiced images for each plot. The experimental work was conducted in a Picea mariana [black spruce] – Sphagnum spp. bog forest in northern Minnesota, 40 km north of Grand Rapids, in the USDA Forest Service Marcell Experimental Forest (MEF). This ecosystem, which is located at the southern margin of the boreal forest, is considered especially vulnerable to climate change and anticipated to be near its tipping point.},

  doi          = {10.25581/spruce.081/1637336},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Wed Jan 01 00:00:00 EST 2020},

  month        = {Wed Jan 01 00:00:00 EST 2020}

}

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DOI: https://doi.org/10.25581/spruce.081/1637336

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High‐resolution minirhizotrons advance our understanding of root‐fungal dynamics in an experimentally warmed peatland

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Mycorrhizal fungi enable plants to thrive in the cold, waterlogged, organic soils of boreal peatlands and, with saprotrophic fungi, largely contribute to the sequestration of atmospheric carbon in peat. Hence, fungi support the contribution of peatlands to global climate regulation, on which society depends. Here we used high-resolution minirhizotrons for an unprecedented glimpse of the belowground world of a forested bog and highlighted linkages between environmental change and the abundance, dynamics, and morphology of vascular plant fine roots and fungal mycelium. These changes may have implications for peat carbon accumulation on the boreal landscape.
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Fine roots contribute to ecosystem carbon, water, and nutrient fluxes through resource acquisition, respiration, exudation, and turnover, but are understudied in peatlands. This data set reports fine-root peak growth and standing crop measurements from a forested, ombrotrophic bog as determined using non-destructive minirhizotron technology. Minirhizotron images were collected throughout the growing seasons of 2011 and 2012 at the southern and northern ends of the S1 bog across gradients of tree density in paired hummock and hollow microtopography. The dominant woody species in the bog, and focus of the investigation, were trees Picea mariana and Larix laricina, and ericaceous shrubs Rhododendronmore » groenlandicum and Chamaedaphne calyculata. The data were derived from minirhizotron images where root length and diameter were measured on ~weekly images and used to calculate: (1) Annual fine-root production and peak standing crop in 2011 and 2012; (2) Seasonal fine-root phenology throughout the growing seasons of 2011 and 2012; and (3) The distribution of newly-produced fine-root length throughout the peat profile in 2011 and 2012. The measurements were made in the S1 Bog, a Picea mariana [black spruce] – Sphagnum spp. bog forest in northern Minnesota, 40 km north of Grand Rapids, in the USDA Forest Service Marcell Experimental Forest (MEF). These minirhizotron images were collected prior to the construction of the SPRUCE experimental plots.« less
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