SPRUCE High-Resolution Minirhizotrons in an Experimentally-Warmed Peatland Provide an Unprecedented Glimpse at Fine Roots and their Fungal Partners: Supporting Data

Childs, J.; Defrenne, C.E.; Brice, D.J.; Woodward, J.; Holbrook, K.N.; Nettles, W.R.; Taggart, M.; Iversen, C.M.

doi:10.25581/spruce.081/1637336

Title: SPRUCE High-Resolution Minirhizotrons in an Experimentally-Warmed Peatland Provide an Unprecedented Glimpse at Fine Roots and their Fungal Partners: Supporting Data

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Abstract

Images were collected using first of their kind, non-destructive, high-resolution automated minirhizotrons (RhizoSystems, LLC) to assess the response of plant fine-root and fungal mycelium dynamics to elevated temperatures after 4-6 years of whole-ecosystem warming and exposure to elevated carbon dioxide concentrations (e[CO2]) in a peat bog where the SPRUCE experiment is located. We focused on two SPRUCE experimental plots: Plot 10 has elevated temperature (+9°C) and plot 19 is a control (+0°C). Both have elevated CO2 (e[CO2]). Changes in root and fungal abundance with warming were estimated from a timeseries of landscape-level mosaiced images for each plot by measuring the proportional abundance of five belowground classes: fine roots of vascular plants, ectomycorrhizas, fungal hyphae, fungal rhizomorphs, and fungal sporocarps. To examine root and fungal phenology responses to warming, the length per individual root or fungal structure areal coverage were measured per image area of a set of timeseries patch-level mosaiced images for each plot. The experimental work was conducted in a Picea mariana [black spruce] – Sphagnum spp. bog forest in northern Minnesota, 40 km north of Grand Rapids, in the USDA Forest Service Marcell Experimental Forest (MEF). This ecosystem, which is located at the southern margin of the borealmore »« less

Authors:

Childs, J.; Defrenne, C.E.; Brice, D.J.; Woodward, J.; Holbrook, K.N.;

; Taggart, M.;

Publication Date:: 2020-01-01

DOE Contract Number:: AC05-00OR22725

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Earth and Environmental Systems Science Division

Subject:: 54 ENVIRONMENTAL SCIENCES

Keywords:: plant fine-root, fungal mycelium, automated minirhizotrons, abundance, phenology, ectomycorrhizas, fungal hyphae, fungal rhizomorphs, fungal sporocarps, image analyses

Geolocation:: 47.50656,-93.45256|47.5047,-93.45256|47.5047,-93.45399|47.50656,-93.45399|47.50656,-93.45256

OSTI Identifier:: 1637336

DOI:: https://doi.org/10.25581/spruce.081/1637336

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Citation Formats


                    Childs, J., Defrenne, C.E., Brice, D.J., Woodward, J., Holbrook, K.N., Nettles, W.R., Taggart, M., and Iversen, C.M. SPRUCE High-Resolution Minirhizotrons in an Experimentally-Warmed Peatland Provide an Unprecedented Glimpse at Fine Roots and their Fungal Partners: Supporting Data.  United States: N. p., 2020. 
        Web.  doi:10.25581/spruce.081/1637336.

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                    Childs, J., Defrenne, C.E., Brice, D.J., Woodward, J., Holbrook, K.N., Nettles, W.R., Taggart, M., & Iversen, C.M. SPRUCE High-Resolution Minirhizotrons in an Experimentally-Warmed Peatland Provide an Unprecedented Glimpse at Fine Roots and their Fungal Partners: Supporting Data.  United States.  doi:https://doi.org/10.25581/spruce.081/1637336

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                    Childs, J., Defrenne, C.E., Brice, D.J., Woodward, J., Holbrook, K.N., Nettles, W.R., Taggart, M., and Iversen, C.M. 2020.  
"SPRUCE High-Resolution Minirhizotrons in an Experimentally-Warmed Peatland Provide an Unprecedented Glimpse at Fine Roots and their Fungal Partners: Supporting Data".  United States.  doi:https://doi.org/10.25581/spruce.081/1637336.  https://www.osti.gov/servlets/purl/1637336. Pub date:Wed Jan 01 00:00:00 EST 2020

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@article{osti_1637336,

  title        = {SPRUCE High-Resolution Minirhizotrons in an Experimentally-Warmed Peatland Provide an Unprecedented Glimpse at Fine Roots and their Fungal Partners: Supporting Data},

  author       = {Childs, J. and Defrenne, C.E. and Brice, D.J. and Woodward, J. and Holbrook, K.N. and Nettles, W.R. and Taggart, M. and Iversen, C.M.},

  abstractNote = {Images were collected using first of their kind, non-destructive, high-resolution automated minirhizotrons (RhizoSystems, LLC) to assess the response of plant fine-root and fungal mycelium dynamics to elevated temperatures after 4-6 years of whole-ecosystem warming and exposure to elevated carbon dioxide concentrations (e[CO2]) in a peat bog where the SPRUCE experiment is located. We focused on two SPRUCE experimental plots: Plot 10 has elevated temperature (+9°C) and plot 19 is a control (+0°C). Both have elevated CO2 (e[CO2]). Changes in root and fungal abundance with warming were estimated from a timeseries of landscape-level mosaiced images for each plot by measuring the proportional abundance of five belowground classes: fine roots of vascular plants, ectomycorrhizas, fungal hyphae, fungal rhizomorphs, and fungal sporocarps. To examine root and fungal phenology responses to warming, the length per individual root or fungal structure areal coverage were measured per image area of a set of timeseries patch-level mosaiced images for each plot. The experimental work was conducted in a Picea mariana [black spruce] – Sphagnum spp. bog forest in northern Minnesota, 40 km north of Grand Rapids, in the USDA Forest Service Marcell Experimental Forest (MEF). This ecosystem, which is located at the southern margin of the boreal forest, is considered especially vulnerable to climate change and anticipated to be near its tipping point.},

  doi          = {10.25581/spruce.081/1637336},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {2020},

  month        = {1}

}

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DOI: https://doi.org/10.25581/spruce.081/1637336

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High-resolution minirhizotrons advance our understanding of root-fungal dynamics in an experimentally warmed peatland

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