Rare Earth Desorption Test with Citrate and Bicarbonate

Jiao, Yongqin; Brewer, Aaron; Park, Dan

doi:10.15121/1452718

Title: Rare Earth Desorption Test with Citrate and Bicarbonate

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Abstract

LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min. Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.

Authors:

Jiao, Yongqin; Brewer, Aaron; Park, Dan

Lawrence Livermore National Laboratory

Publication Date:: Thu Jun 01 04:00:00 UTC 2017

Other Number(s):: 965

Research Org.:: DOE Geothermal Data Repository; Lawrence Livermore National Laboratory

Sponsoring Org.:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Geothermal Technologies Program (EE-2C)

Collaborations:: Lawrence Livermore National Laboratory

Subject:: 15 GEOTHERMAL ENERGY; REE; adsorption; bicarbonate; brine; centrifuge; citrate; desorption; energy; geothermal; lanthanide binding tag; lbt; rare earth; resource recovery

OSTI Identifier:: 1452718

DOI:: https://doi.org/10.15121/1452718

Citation Formats


                    Jiao, Yongqin, Brewer, Aaron, and Park, Dan. Rare Earth Desorption Test with Citrate and Bicarbonate.  United States: N. p., 2017. 
        Web.  doi:10.15121/1452718.

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                    Jiao, Yongqin, Brewer, Aaron, & Park, Dan. Rare Earth Desorption Test with Citrate and Bicarbonate.  United States.  doi:https://doi.org/10.15121/1452718

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                    Jiao, Yongqin, Brewer, Aaron, and Park, Dan. 2017.  
"Rare Earth Desorption Test with Citrate and Bicarbonate".  United States.  doi:https://doi.org/10.15121/1452718.  https://www.osti.gov/servlets/purl/1452718. Pub date:Thu Jun 01 04:00:00 UTC 2017

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@article{osti_1452718,

  title        = {Rare Earth Desorption Test with Citrate and Bicarbonate},

  author       = {Jiao, Yongqin and Brewer, Aaron and Park, Dan},

  abstractNote = {LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min. Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.},

  doi          = {10.15121/1452718},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Thu Jun 01 04:00:00 UTC 2017},

  month        = {Thu Jun 01 04:00:00 UTC 2017}

}

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DOI: https://doi.org/10.15121/1452718

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