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Title: Rare Earth Desorption Test with Citrate and Bicarbonate

Abstract

LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min. Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.

Authors:
; ;
Publication Date:
Other Number(s):
965
DOE Contract Number:  
LLNL FY17 AOP 2.5.1.12
Product Type:
Dataset
Research Org.:
DOE Geothermal Data Repository; Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Geothermal Technologies Program (EE-2C)
Subject:
15 Geothermal Energy
Keywords:
geothermal; energy; adsorption; desorption; rare earth; citrate; bicarbonate; REE; resource recovery; centrifuge; brine; lbt; lanthanide binding tag
OSTI Identifier:
1452718
DOI:
10.15121/1452718

Citation Formats

Jiao, Yongqin, Brewer, Aaron, and Park, Dan. Rare Earth Desorption Test with Citrate and Bicarbonate. United States: N. p., 2017. Web. doi:10.15121/1452718.
Jiao, Yongqin, Brewer, Aaron, & Park, Dan. Rare Earth Desorption Test with Citrate and Bicarbonate. United States. doi:10.15121/1452718.
Jiao, Yongqin, Brewer, Aaron, and Park, Dan. 2017. "Rare Earth Desorption Test with Citrate and Bicarbonate". United States. doi:10.15121/1452718. https://www.osti.gov/servlets/purl/1452718. Pub date:Thu Jun 01 00:00:00 EDT 2017
@article{osti_1452718,
title = {Rare Earth Desorption Test with Citrate and Bicarbonate},
author = {Jiao, Yongqin and Brewer, Aaron and Park, Dan},
abstractNote = {LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min. Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.},
doi = {10.15121/1452718},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {6}
}

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