Design, construction, characterization, and application of a hyperspectral microarray scanner.
- University of New Mexico, Albuquerque, NM
We describe the design, construction, and operation of a hyperspectral microarray scanner for functional genomic research. The hyperspectral instrument operates with spatial resolutions ranging from 3 to 30 {micro}m and records the emission spectrum between 490 and 900 nm with a spectral resolution of 3 nm for each pixel of the microarray. This spectral information, when coupled with multivariate data analysis techniques, allows for identification and elimination of unwanted artifacts and greatly improves the accuracy of microarray experiments. Microarray results presented in this study clearly demonstrate the separation of fluorescent label emission from the spectrally overlapping emission due to the underlying glass substrate. We also demonstrate separation of the emission due to green fluorescent protein expressed by yeast cells from the spectrally overlapping autofluorescence of the yeast cells and the growth media.
- Research Organization:
- Sandia National Laboratories
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC04-94AL85000
- OSTI ID:
- 993916
- Report Number(s):
- SAND2003-2452J
- Journal Information:
- Proposed for publication in Applied Optics., Journal Name: Proposed for publication in Applied Optics. Journal Issue: 10 Vol. 43; ISSN 0003-6935; ISSN APOPAI
- Country of Publication:
- United States
- Language:
- English
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