Fourier analysis and synthesis tomography.

Technical Report · Sat May 01 04:00:00 EDT 2010

DOI:https://doi.org/10.2172/992332· OSTI ID:992332

Wagner, Kelvin H ^[1]; Sinclair, Michael B; Feldkuhn, Daniel ^[1]

---

1. University of Colorado at Boulder, Boulder, CO

Most far-field optical imaging systems rely on a lens and spatially-resolved detection to probe distinct locations on the object. We describe and demonstrate a novel high-speed wide-field approach to imaging that instead measures the complex spatial Fourier transform of the object by detecting its spatially-integrated response to dynamic acousto-optically synthesized structured illumination. Tomographic filtered backprojection is applied to reconstruct the object in two or three dimensions. This technique decouples depth-of-field and working-distance from resolution, in contrast to conventional imaging, and can be used to image biological and synthetic structures in fluoresced or scattered light employing coherent or broadband illumination. We discuss the electronically programmable transfer function of the optical system and its implications for imaging dynamic processes. Finally, we present for the first time two-dimensional high-resolution image reconstructions demonstrating a three-orders-of-magnitude improvement in depth-of-field over conventional lens-based microscopy.

• Research Organization: Sandia National Laboratories
• Sponsoring Organization: USDOE
• DOE Contract Number: AC04-94AL85000
• OSTI ID: 992332
• Report Number(s): SAND2010-3258
• Country of Publication: United States
• Language: English

Related Subjects

47 OTHER INSTRUMENTATION
FOURIER ANALYSIS
ILLUMINANCE
Microscopy-Technique.
OPTICAL SYSTEMS
Optical instruments-Testing.
Optical materials.
Optical tomography
PERFORMANCE
RESOLUTION
TOMOGRAPHY
TRANSFER FUNCTIONS