Microbial dynamics in upflow anaerobic sludge blanket (UASB) bioreactor granules in response to short-term changes in substrate feed
The complexity and diversity of the microbial communities in biogranules from an upflow anaerobic sludge blanket (UASB) bioreactor were determined in response to short-term changes in substrate feeds. The reactor was fed simulated brewery wastewater (SBWW) (70% ethanol, 15% acetate, 15% propionate) for 1.5 months (phase 1), acetate / sulfate for 2 months (phase 2), acetate-alone for 3 months (phase 3), and then a return to SBWW for 2 months (phase 4). Performance of the reactor remained relatively stable throughout the experiment as shown by COD removal and gas production. 16S rDNA, methanogen-associated mcrA and sulfate reducer-associated dsrAB genes were PCR amplified, then cloned and sequenced. Sequence analysis of 16S clone libraries showed a relatively simple community composed mainly of the methanogenic Archaea (Methanobacterium and Methanosaeta), members of the Green Non-Sulfur (Chloroflexi) group of Bacteria, followed by fewer numbers of Syntrophobacter, Spirochaeta, Acidobacteria and Cytophaga-related Bacterial sequences. Methanogen-related mcrA clone libraries were dominated throughout by Methanobacter and Methanospirillum related sequences. Although not numerous enough to be detected in our 16S rDNA libraries, sulfate reducers were detected in dsrAB clone libraries, with sequences related to Desulfovibrio and Desulfomonile. Community diversity levels (Shannon-Weiner index) generally decreased for all libraries in response to a change from SBWW to acetate-alone feed. But there was a large transitory increase noted in 16S diversity at the two-month sampling on acetate-alone, entirely related to an increase in Bacterial diversity. Upon return to SBWW conditions in phase 4, all diversity measures returned to near phase 1 levels.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 991574
- Report Number(s):
- PNNL-SA-47879; TRN: US201021%%212
- Journal Information:
- Microbiology, 156(Pt. 8):2418-2427, Vol. 156, Issue 8
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BACTERIA
BIOREACTORS
COMMUNITIES
DESULFOVIBRIO
ETHANOL
GENES
PERFORMANCE
PRODUCTION
REMOVAL
SAMPLING
SLUDGES
STRUCTURAL CHEMICAL ANALYSIS
SUBSTRATES
SULFATES
16S RIBOSOMAL-RNA
POLYMERASE-CHAIN-REACTION
CONFORMATION POLYMORPHISM ANALYSIS
SP-NOV
SYNTROPHIC ASSOCIATION
METHANOGENIC COMMUNITY
OXIDIZING BACTERIUM
ACETATE OXIDATION
WASTE-WATER
GEN. NOV.