Separation mechanism of nortriptyline and amytriptyline in RPLC
- University of Tennessee, Knoxville (UTK)
- ORNL
The single and the competitive equilibrium isotherms of nortriptyline and amytriptyline were acquired by frontal analysis (FA) on the C{sub 18}-bonded discovery column, using a 28/72 (v/v) mixture of acetonitrile and water buffered with phosphate (20 mM, pH 2.70). The adsorption energy distributions (AED) of each compound were calculated from the raw adsorption data. Both the fitting of the adsorption data using multi-linear regression analysis and the AEDs are consistent with a trimodal isotherm model. The single-component isotherm data fit well to the tri-Langmuir isotherm model. The extension to a competitive two-component tri-Langmuir isotherm model based on the best parameters of the single-component isotherms does not account well for the breakthrough curves nor for the overloaded band profiles measured for mixtures of nortriptyline and amytriptyline. However, it was possible to derive adjusted parameters of a competitive tri-Langmuir model based on the fitting of the adsorption data obtained for these mixtures. A very good agreement was then found between the calculated and the experimental overloaded band profiles of all the mixtures injected.
- Research Organization:
- Oak Ridge National Laboratory (ORNL)
- Sponsoring Organization:
- SC USDOE - Office of Science (SC)
- DOE Contract Number:
- AC05-00OR22725
- OSTI ID:
- 989567
- Journal Information:
- Journal of Chromatography A, Journal Name: Journal of Chromatography A Journal Issue: 2005 Vol. 1090; ISSN 0021-9673
- Country of Publication:
- United States
- Language:
- English
Similar Records
Isotherm parameters and intraparticle mass transfer kinetics on molecularly imprinted polymers in acetonitrile/buffer mobile phases
Adsorption mechanism in RPLC. Effect of the nature of the organic modifier
Related Subjects
ADSORPTION ISOTHERMS
ANTIDEPRESSANTS
LIQUID COLUMN CHROMATOGRAPHY
REGRESSION ANALYSIS
acetonitrile
adsorption equilibrium
affinity energy distribution
amytriptyline
column heterogeneity
discovery-C18
frontal analysis
nortriptyline
preparative chromatography
retention mechanism
silica
single and competitive adsorption isotherms