The genome sequence of the obligately chemolithoautotrophic, facultatively anaerobic bacterium Thiobacillus denitfificans.

Journal Article · Wed Feb 01 04:00:00 EST 2006 · Journal of Bacteriology

DOI:https://doi.org/10.1128/JB.188.4.1473-1488.2006· OSTI ID:989527

Beller, H R ^[1]; Larimer, Frank W ^[2]

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1. Lawrence Livermore National Laboratory (LLNL)
2. ORNL

The complete genome sequence of Thiobacillus denitrificans ATCC 25259 is the first to become available for an obligately chemolithoautotrophic, sulfur-compound-oxidizing, {beta}-proteobacterium. Analysis of the 2,909,809-bp genome will facilitate our molecular and biochemical understanding of the unusual metabolic repertoire of this bacterium, including its ability to couple denitrification to sulfur-compound oxidation, to catalyze anaerobic, nitrate-dependent oxidation of Fe(II) and U(IV), and to oxidize mineral electron donors. Notable genomic features include (i) genes encoding c-type cytochromes totaling 1 to 2 percent of the genome, which is a proportion greater than for almost all bacterial and archaeal species sequenced to date, (ii) genes encoding two [NiFe]hydrogenases, which is particularly significant because no information on hydrogenases has previously been reported for T. denitrificans and hydrogen oxidation appears to be critical for anaerobic U(IV) oxidation by this species, (iii) a diverse complement of more than 50 genes associated with sulfur-compound oxidation (including sox genes, dsr genes, and genes associated with the AMP-dependent oxidation of sulfite to sulfate), some of which occur in multiple (up to eight) copies, (iv) a relatively large number of genes associated with inorganic ion transport and heavy metal resistance, and (v) a paucity of genes encoding organic-compound transporters, commensurate with obligate chemolithoautotrophy. Ultimately, the genome sequence of T. denitrificans will enable elucidation of the mechanisms of aerobic and anaerobic sulfur-compound oxidation by {beta}-proteobacteria and will help reveal the molecular basis of this organism's role in major biogeochemical cycles (i.e., those involving sulfur, nitrogen, and carbon) and groundwater restoration.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: SC USDOE - Office of Science (SC)
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 989527
• Journal Information: Journal of Bacteriology, Journal Name: Journal of Bacteriology Journal Issue: 4 Vol. 188; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

08 HYDROGEN
BINDING ENERGY
CARBON
CYTOCHROMES
DENITRIFICANS
DENITRIFICATION
ELECTRONS
ESCHERICHIA-COLI
FLAVOPROTEIN SOXF
GENE-CLUSTER
GENES
HEAVY METALS
HYDROGEN
HYDROGENASES
INORGANIC SULFUR-COMPOUNDS
INTRACELLULAR POLYGLUCOSE
NITROGEN
NITROSOMONAS-EUROPAEA
OXIDATION
PARACOCCUS-PANTOTROPHUS GB17
SIROHEME SULFITE REDUCTASE
SULFITES
SULFUR
THIOSULFATE-OXIDIZING BACTERIA
TRANSPORT
VALENCE