Aptamer/Graphene Oxide Nanocomplex for In Situ Molecular Probing in Living Cells

Journal Article · Mon Jun 21 04:00:00 EDT 2010 · Journal of the American Chemical Society, 132(27):9274–9276

DOI:https://doi.org/10.1021/ja103169v· OSTI ID:988651

Wang, Ying; Li, Zhaohui; Hu, Dehong; Lin, Chiann Tso; Li, Jinghong; Lin, Yuehe

In this communication, we report the illustration of cellular delivering and in situ molecular probing in living cells by using graphene oxide nanosheets (GO-nS) as DNA cargo and sensing platform. Due to the particular interaction between GO and DNA molecules, aptamer/GO-nS nanocomplex was designed and employed to demonstrate the dramatic DNA delivering, enzymatic cleavage protecting and biosensing capabilities of GO-nS in living cells. The results show that GO-nS could successfully transfer DNA aptamer into living cells, efficiently protect oligonucleotides from enzymatic cleavage during the delivery as well as selectively monitoring intra-cellular target in situ. The as-shown advantages of graphene oxide will enable it to be an robust candidate for many biological fields, such as DNA and protein analyzing, gene and drug delivering, intracellular tracking, and in vivo monitoring, etc..

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 988651
• Report Number(s): PNNL-SA-73086
• Journal Information: Journal of the American Chemical Society, 132(27):9274–9276, Journal Name: Journal of the American Chemical Society, 132(27):9274–9276 Journal Issue: 27 Vol. 132; ISSN JACSAT; ISSN 0002-7863
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS
CARGO
CLEAVAGE
Cellular imaging
Graphene
ATP
Fluorescence
DNA
GENES
IN VIVO
MONITORING
OLIGONUCLEOTIDES
OXIDES
PROTEINS
TARGETS