Minimizing analyte electrolysis in electrospray ionization mass spectrometry using a redox buffer coated emitter electrode

Journal Article · Fri Jan 01 04:00:00 EST 2010 · Rapid Communications in Mass Spectrometry

DOI:https://doi.org/10.1002/rcm.4522· OSTI ID:988256

Peintler-Krivan, Emese ^[1]; Van Berkel, Gary J ^[1]; Kertesz, Vilmos ^[1]

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1. ORNL

An emitter electrode with an electroactive poly(pyrrole) (PPy) polymer film coating was constructed for use in electrospray ionization mass spectrometry (ESI-MS). The PPy film acted as a surface-attached redox buffer limiting the interfacial potential of the emitter electrode. While extensive oxidation of selected analytes (reserpine and amodiaquine) was observed in positive ion mode ESI using a bare metal (gold) emitter electrode, the oxidation was suppressed for these same analytes when using the PPy-coated electrode. A semi-quantitative relationship between the rate of oxidation observed and the interfacial potential of the emitter electrode was shown. The redox buffer capacity, and therefore the lifetime of the redox buffering effect, correlated with the oxidation potential of the analyte and with the magnitude of the film charge capacity. Online reduction of the PPy polymer layer using negative ion mode ESI between analyte injections was shown to successfully restore the redox buffering capacity of the polymer film to its initial state.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: ORNL work for others
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 988256
• Journal Information: Rapid Communications in Mass Spectrometry, Journal Name: Rapid Communications in Mass Spectrometry Journal Issue: 9 Vol. 24; ISSN 0951-4198; ISSN 1097-0231
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
BUFFERS
COATINGS
ELECTRODES
ELECTROLYSIS
GOLD
IONIZATION
MASS SPECTROSCOPY
MITIGATION
OXIDATION
POLYMERS
PYRROLES
RESERPINE