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Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum

Patent ·
OSTI ID:986100
 [1];  [1];  [2];  [1];  [3];  [1]
  1. Golden, CO
  2. Berthoud, CO
  3. Littleton, CO
+ Show Author Affiliations

Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC36-99GO10337
Assignee:
Alliance For Sustainable Energy, LLC (Golden, CO)
Patent Number(s):
7,449,550
Application Number:
10/557,589
OSTI ID:
986100
Country of Publication:
United States
Language:
English

References (9)

Simultaneous saccharification and fermentation (SSF) of lignocellulosics to ethanol under vacuum cycling and step feeding journal April 1984
Synthetic Oligodeoxynucleotides for Analyses of DNA Structure and Function book January 1978
Characterization of the major endo-1,4-β-d-glucanases from the cellulase of Penicillium pinophilum/funiculosum journal February 1989
Family 7 cellobiohydrolases from Phanerochaete chrysosporium: crystal structure of the catalytic module of Cel7D (CBH58) at 1.32 Å resolution and homology models of the isozymes journal December 2001
Use of a New Membrane-Reactor Saccharification Assay to Evaluate the Performance of Cellulases Under Simulated SSF Conditions book January 1997
The isolation, purification and properties of the cellobiohydrolase component of Penicillium funiculosum cellulase journal July 1980
Evaluation of the cellobiose-fermenting yeastBrettanomyces custersii in the simultaneous saccharification and fermentation of cellulose journal May 1992
The characterization of a commercial cellulase product from Penicillium funiculosum by fast protein liquid chromatography journal April 1985
Hydrolysis of cellulose using ternary mixtures of purified celluloses journal March 1998

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Related Subjects

09 BIOMASS FUELS
AGRICULTURAL WASTES
ASPERGILLUS
CELLULASE
ENZYMES
HYDROLASES
MAIZE
PENICILLIUM
PERFORMANCE
TRICHODERMA VIRIDE