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Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs

Patent ·
OSTI ID:985668
 [1];  [2];  [2];  [3];  [4];  [5];  [6];  [7];  [2];  [8];  [2]
  1. La Jolla, CA
  2. San Diego, CA
  3. Cambridge, GB
  4. Lexington, MA
  5. North Haven, CT
  6. Philadelphia, PA
  7. Lancaster, PA
  8. Cambridge, MA
+ Show Author Affiliations

This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.

Research Organization:
The Scripps Research Institute (La Jolla, CA)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG03-00ER45812
Assignee:
The Scripps Research Institute (La Jolla, CA); The Regents of the University of California (Oakland, CA)
Patent Number(s):
7,354,761
Application Number:
11/254,170
OSTI ID:
985668
Country of Publication:
United States
Language:
English

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