Laser-induced Breakdown Spectroscopy used to Detect Endophyte-mediated Accumulation of Metals by Tall Fescue

Journal Article · Fri Jan 01 00:00:00 EST 2010 · Applied Optics

DOI:https://doi.org/10.1364/AO.49.00C161· OSTI ID:985286

Martin, Madhavi Z ^[1]; Stewart, Arthur J ^[1]; Gwinn, Dr. Kimberley ^[2]; Waller, John C ^[1]

---

1. ORNL
2. University of Tennessee, Knoxville (UTK)

Laser-induced breakdown spectroscopy was used to determine the impact of endophyte (Neotyphodium sp.) infection on elemental composition of tall fescue (Festuca arundinacea). Leaf material from endophyte-infected (E+) and endophyte-free (E-) tall fescue populations in established plots was examined. Leaf-tissue digestates were also tested for metals, by ICP-MS. Seven of eleven metals (Ca, Mg, Fe, Mn, Cu, Ni and Zn) were measured by both techniques at concentrations great enough to reliably compare. Mg, Zn, and Cd, a toxic metal that can be present in forage, were readily detected by LIBS, even though Cd concentrations in the plants were below levels typically achieved using ICP-MS detection. Implications of these results for research on forage analysis and phytoremediation are discussed.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 985286
• Journal Information: Applied Optics, Vol. 49, Issue 13; ISSN 0003-6935
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
54 ENVIRONMENTAL SCIENCES
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
BIOLOGICAL ACCUMULATION
METALS
DETECTION
LASER SPECTROSCOPY
GRAMINEAE
MULTI-ELEMENT ANALYSIS
MASS SPECTROSCOPY
FORAGE
REMEDIAL ACTION
LIBS
metal detection in plants
endophyte mediated
metal accumulation