Laser-induced Breakdown Spectroscopy used to Detect Endophyte-mediated Accumulation of Metals by Tall Fescue
- ORNL
- University of Tennessee, Knoxville (UTK)
Laser-induced breakdown spectroscopy was used to determine the impact of endophyte (Neotyphodium sp.) infection on elemental composition of tall fescue (Festuca arundinacea). Leaf material from endophyte-infected (E+) and endophyte-free (E-) tall fescue populations in established plots was examined. Leaf-tissue digestates were also tested for metals, by ICP-MS. Seven of eleven metals (Ca, Mg, Fe, Mn, Cu, Ni and Zn) were measured by both techniques at concentrations great enough to reliably compare. Mg, Zn, and Cd, a toxic metal that can be present in forage, were readily detected by LIBS, even though Cd concentrations in the plants were below levels typically achieved using ICP-MS detection. Implications of these results for research on forage analysis and phytoremediation are discussed.
- Research Organization:
- Oak Ridge National Laboratory (ORNL)
- Sponsoring Organization:
- SC USDOE - Office of Science (SC)
- DOE Contract Number:
- AC05-00OR22725
- OSTI ID:
- 985286
- Journal Information:
- Applied Optics, Journal Name: Applied Optics Journal Issue: 13 Vol. 49; ISSN 0003-6935; ISSN 1539-4522
- Country of Publication:
- United States
- Language:
- English
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