Nanobiotechnology for enzymatic remediation and soil carbon sequestration
Conference
·
OSTI ID:985085
We studied the ability of tyrosinase to catalyze the oxidation of various phenolic compounds. As a revolutionary approach to enzyme stabilization, we developed specially-designed nanoporous silica for enzyme immobilization. Our tests show that the active lifetime of the enzymes stabilized in this material can extend to periods as long as several months, which is about a 100-fold increase in stability. The implications of this new approach to enzyme-based bioremedation will be discussed. In soils, the humification process involves phenol oxidation, mediated by tyrosinase, followed by nonenzymatic polymerization of the resulting quinones with amino acids to form humic polymers. We tested the effects of fly ash amendments on a model humification reaction involving tyrosinase and a suite of organic monomers. The combination of fly ashes with tyrosinase increased the amount of polymer formed by several fold. The strong synergetic effect of these ashes when enzyme is present apparently arises from the combined effects of alkaline pH and physical stabilization of the enzyme in porous silica cenospheres.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 985085
- Report Number(s):
- PNNL-SA-43410; KP1301020
- Country of Publication:
- United States
- Language:
- English
Similar Records
Fly Ash Amendments Catalyze Soil Carbon Sequestration
Effect of humic constituents on the transformation of chlorinated phenols and anilines in the presence of oxidoreductive enzymes or birnessite
Tyrosinase-containing chitosan gels: A combined catalyst and sorbent for selective phenol removal
Conference
·
Mon Sep 15 00:00:00 EDT 2003
·
OSTI ID:981614
Effect of humic constituents on the transformation of chlorinated phenols and anilines in the presence of oxidoreductive enzymes or birnessite
Journal Article
·
Tue Jun 15 00:00:00 EDT 1999
· Environmental Science and Technology
·
OSTI ID:6335403
Tyrosinase-containing chitosan gels: A combined catalyst and sorbent for selective phenol removal
Journal Article
·
Fri Jul 05 00:00:00 EDT 1996
· Biotechnology and Bioengineering
·
OSTI ID:260440