Deposition of Lignin Droplets Produced During Dilute Acid Pretreatment of Maize Stems Retards Enzymatic Hydrolysis of Cellulose
Electron microscopy of lignocellulosic biomass following high-temperature pretreatment revealed the presence of spherical formations on the surface of the residual biomass. The hypothesis that these droplet formations are composed of lignins and possible lignin carbohydrate complexes is being explored. Experiments were conducted to better understand the formation of these lignin droplets and the possible implications they might have on the enzymatic saccharification of pretreated biomass. It was demonstrated that these droplets are produced from corn stover during pretreatment under neutral and acidic pH at and above 130C, and that they can deposit back onto the surface of residual biomass. The deposition of droplets produced under certain pretreatment conditions (acidic pH; T > 150C) and captured onto pure cellulose was shown to have a negative effect (5-20%) on the enzymatic saccharification of this substrate. It was noted that droplet density (per unit area) was greater and droplet size more variable under conditions where the greatest impact on enzymatic cellulose conversion was observed. These results indicate that this phenomenon has the potential to adversely affect the efficiency of enzymatic conversion in a lignocellulosic biorefinery.
- Research Organization:
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC36-08GO28308
- OSTI ID:
- 982273
- Journal Information:
- Biotechnology Progress, Vol. 23, Issue 6, 2007; ISSN 8756-7938
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AGRICULTURAL WASTES
BIOMASS
CARBOHYDRATES
CELLULOSE
COMPLEXES
CONVERSION
DENSITY
DEPOSITION
DEPOSITS
DROPLETS
ELECTRON MICROSCOPY
ENZYMATIC HYDROLYSIS
HYPOTHESIS
LIGNIN
MAIZE
POTENTIALS
SACCHARIFICATION
SIZE
SURFACES
TEMPERATURE RANGE 0400-1000 K
Chemical and Biosciences
Bioenergy