The role of Shewanella oneidensis MR-1 outer surface structures in extracellular electron transfer
Shewanella oneidensis is a facultative anaerobe that uses more than 14 different terminal electron acceptors for respiration. These include metal oxides and hydroxyoxides, and toxic metals such as uranium and chromium. Mutants deficient in metal reduction were isolated using the mariner transposon derivative, minihimar RB1. These included mutants with transposon insertions in the prepilin peptidase and type II secretion system genes. All mutants were deficient in Fe(III) and Mn(IV) reduction, and exhibited slow growth when DMSO was used as the electron acceptor. The genome sequence of S. oneidensis contains one prepilin peptidase gene, pilD. A similar prepilin peptidase that may function in the processing of type II secretion prepilins was not found. Single and multiple chromosomal deletions of four putative type IV pilin genes did not affect Fe(III) and Mn(IV) reduction. These results indicate that PilD in S. oneidensis is responsible for processing both type IV and type II secretion prepilin proteins. Type IV pili do not appear to be required for Fe(III) and Mn(IV) reduction.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 981994
- Report Number(s):
- PNNL-SA-52140
- Journal Information:
- Electroanalysis, 22(7-8):856-864, Journal Name: Electroanalysis, 22(7-8):856-864 Journal Issue: 7-8 Vol. 22; ISSN 1521-4109; ISSN 1040-0397
- Country of Publication:
- United States
- Language:
- English
Similar Records
Current Production and Metal Oxide Reduction by Shewanella Oneidensis MR-1 Wild Type and Mutants.
Current production and metal oxide reduction by Shewanella oneidensis MR-1 wild type and mutants (vol 73, pg 7003, 2007)