The role of Shewanella oneidensis MR-1 outer surface structures in extracellular electron transfer

Journal Article · Tue Apr 20 00:00:00 EDT 2010 · Electroanalysis, 22(7-8):856-864

DOI:https://doi.org/10.1002/elan.200880006· OSTI ID:981994

Bouhenni, Rachida; Vora, Gary J; Biffinger, Justin C; Shirodkar, Sheetal; Brockman, K L; Ray, Ricky; Wu, Peter; Johnson, Brandy J; Biddle, E M; Marshall, Matthew J; Fitzgerald, Lisa A; Little, Brenda; Fredrickson, Jim K; Beliaev, Alex S; Ringeisen, Bradley R; Saffarini, Daad

Shewanella oneidensis is a facultative anaerobe that uses more than 14 different terminal electron acceptors for respiration. These include metal oxides and hydroxyoxides, and toxic metals such as uranium and chromium. Mutants deficient in metal reduction were isolated using the mariner transposon derivative, minihimar RB1. These included mutants with transposon insertions in the prepilin peptidase and type II secretion system genes. All mutants were deficient in Fe(III) and Mn(IV) reduction, and exhibited slow growth when DMSO was used as the electron acceptor. The genome sequence of S. oneidensis contains one prepilin peptidase gene, pilD. A similar prepilin peptidase that may function in the processing of type II secretion prepilins was not found. Single and multiple chromosomal deletions of four putative type IV pilin genes did not affect Fe(III) and Mn(IV) reduction. These results indicate that PilD in S. oneidensis is responsible for processing both type IV and type II secretion prepilin proteins. Type IV pili do not appear to be required for Fe(III) and Mn(IV) reduction.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 981994
• Report Number(s): PNNL-SA-52140; ISSN 1521-4109; TRN: US201012%%1430
• Journal Information: Electroanalysis, 22(7-8):856-864, Vol. 22, Issue 7-8; ISSN 1040-0397
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
BINDING ENERGY
CHROMIUM
DMSO
ELECTRON TRANSFER
ELECTRONS
FUNCTIONS
GENES
GROWTH
METALS
MUTANTS
OXIDES
PROCESSING
PROTEINS
REDUCTION
RESPIRATION
SECRETION
SURFACES
TRANSPOSONS
URANIUM
VALENCE