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Complete genome sequence of the Medicago microsymbiont Ensifer (Sinorhizobium) medicae strain WSM419

Journal Article · · Standards in Genomic Sciences
DOI:https://doi.org/10.4056/sigs.43526· OSTI ID:981790
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  1. Murdoch Univ., Perth (Australia)
  2. DOE Joint Genome Institute, Walnut Creek, CA (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  3. DOE Joint Genome Institute, Walnut Creek, CA (United States)
  4. DOE Joint Genome Institute, Walnut Creek, CA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  5. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  6. Murdoch Univ., Perth (Australia); Department of Agriculture and Food, South Perth (Australia)
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Ensifer (Sinorhizobium) medicae is an effective nitrogen fixing microsymbiont of a diverse range of annual Medicago (medic) species. Strain WSM419 is an aerobic, motile, non-spore forming, Gram-negative rod isolated from a M. murex root nodule collected in Sardinia, Italy in 1981. WSM419 was manufactured commercially in Australia as an inoculant for annual medics during 1985 to 1993 due to its nitrogen fixation, saprophytic competence and acid tolerance properties. Here we describe the basic features of this organism, together with the complete genome sequence, and annotation. This is the first report of a complete genome sequence for a microsymbiont of the group of annual medic species adapted to acid soils. We reveal that its genome size is 6,817,576 bp encoding 6,518 protein-coding genes and 81 RNA only encoding genes. The genome contains a chromosome of size 3,781,904 bp and 3 plasmids of size 1,570,951 bp, 1,245,408 bp and 219,313 bp. Furthermore, the smallest plasmid is a feature unique to this medic microsymbiont.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
ORNL work for others; USDOE
DOE Contract Number:
AC05-00OR22725
OSTI ID:
981790
Journal Information:
Standards in Genomic Sciences, Journal Name: Standards in Genomic Sciences Journal Issue: 1 Vol. 2; ISSN 1944-3277
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Alphaproteobacteria
Gram-negative rod
aerobic
microsymbiont
nitrogen fixation
non-pathogenic
root-nodule bacteria