Caldicellulosiruptor obsidiansis sp. nov., an anaerobic, extremely thermophilic, cellulolytic bacterium isolated from Obsidian Pool, Yellowstone National Park

Journal Article · Mon Feb 01 00:00:00 EST 2010 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/AEM.01903-09· OSTI ID:981417

Hamilton-Brehm, Scott D. ^[1]; Elkins, James G. ^[1]; Phelps, Tommy Joe ^[1]; Keller, Martin ^[1]; Carroll, Sue L. ^[1]; Allman, Steve L. ^[1]; Podar, Mircea ^[1]; Mosher, Jennifer J. ^[1]; Vishnivetskaya, Tatiana A. ^[1]

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1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Here, a novel, obligately anaerobic, extremely thermophilic, cellulolytic bacterium, designated OB47^T, was isolated from Obsidian Pool, Yellowstone National Park, WY, USA. The isolate was a non-motile, non-spore forming, Gram-positive rod approximately 2 μm long by 0.2 μm wide and grew at temperatures between 55-85oC with the optimum at 78oC. The pH range for growth was 6.0-8.0 with values of near 7.0 being optimal. Growth on cellobiose produced the fastest specific growth rates at 0.75 hr^-1. The organism also displayed fermentative growth on glucose, maltose, arabinose, fructose, starch, lactose, mannose, sucrose, galactose, xylose, arabinogalactan, Avicel, xylan, filter paper, processed cardboard, pectin, dilute acid-pretreated switchgrass and Populus. OB47^T was unable to grow on mannitol, fucose, lignin, Gelrite, acetate, glycerol, ribose, sorbital, carboxymethylcellulose and casein. Yeast extract stimulated growth and thiosulfate, sulfate, nitrate, and sulfur were not reduced. Fermentation end products were mainly acetate, H₂, and CO₂ although lactate and ethanol were produced in 5 l batch fermentations. The G+C content of the DNA was 35 mol% and sequence analysis of the small subunit ribosomal RNA gene placed OB47^T within the genus Caldicellulosiruptor. Based on its phylogenetic and phenotypic properties, the isolate is proposed to be designated Caldicellulosiruptor obsidiansis sp. nov. and OB47T is the type stain (ATCC BAA-2073).

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 981417
• Journal Information: Applied and Environmental Microbiology, Vol. 76, Issue 4; ISSN 0099-2240
• Publisher: American Society for Microbiology
• Country of Publication: United States
• Language: English

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