Protein encapsulated magnetic carriers for micro/nanoscale drug delivery systems.
Novel methods for drug delivery may be based on nanotechnology using non-invasive magnetic guidance of drug loaded magnetic carriers to the targeted site and thereafter released by external ultrasound energy. The key building block of this system is to successfully synthesize biodegradable, magnetic drug carriers. Magnetic carriers using poly(D,L-lactide-co-glycolide) (PLGA) or poly(lactic acid)-poly(ethylene glycol) (PLA-PEG) as matrix materials were loaded with bovine serum albumin (BSA) by a double-emulsion technique. BSA-loaded magnetic microspheres were characterized for size, morphology, surface charge, and magnetization. The BSA encapsulation efficiency was determined by recovering albumin from the microspheres using dimethyl sulfoxide and 0.05N NaOH/0.5% SDS then quantifying with the Micro-BCA protein assay. BSA release profiles were also determined by the Micro-BCA protein assay. The microspheres had drug encapsulation efficiencies up to 90% depending on synthesis parameters. Particles were spherical with a smooth or porous surface having a size range less than 5 {mu}m. The surface charge (expressed as zeta potential) was near neutral, optimal for prolonged intravascular survival. The magnetization of these BSA loaded magnetic carriers was 2 to 6 emu/g, depending on the specific magnetic materials used during synthesis.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Organization:
- Defense Advanced Research Projects Agency (DARPA); Univer. of Chicago Brain Research; Cancer Research Foundations
- DOE Contract Number:
- DE-AC02-06CH11357
- OSTI ID:
- 981304
- Report Number(s):
- ANL/CMT/CP-58154; TRN: US201012%%1288
- Resource Relation:
- Conference: Conference on Microtechnologies in Medicine and Biology; May 12, 2005 - May 15, 2005; Kahuku, HI
- Country of Publication:
- United States
- Language:
- ENGLISH
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Related Subjects
77 NANOSCIENCE AND NANOTECHNOLOGY
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
ALBUMINS
BIOLOGY
CATTLE
DMSO
EFFICIENCY
ENCAPSULATION
MAGNETIC MATERIALS
MAGNETIZATION
MATRIX MATERIALS
MEDICINE
MICROSPHERES
MORPHOLOGY
NANOSTRUCTURES
PROTEINS
SYNTHESIS
TECHNOLOGY UTILIZATION