Telomere sister chromatid exchange in telomerase deficient murine cells
- ORNL
We have recently demonstrated that several types of genomic rearrangements (i.e., telomere sister chromatid exchange (T-SCE), genomic-SCE, or end-to-end fusions) were more often detected in long-term cultured murine telomerase deficient embryonic stem (ES) cells than in freshly prepared murine splenocytes, even through they possessed similar frequencies of critically short telomeres. The high rate of genomic rearrangements in telomerase deficient ES cells, when compared to murine splenocytes, may reflect the cultured cells' gained ability to protect chromosome ends with eroded telomeres allowing them to escape 'end crisis'. However, the possibility that ES cells were more permissive to genomic rearrangements than other cell types or that differences in the microenvironment or genetic background of the animals might consequentially determine the rate of T-SCEs or other genomic rearrangements at critically short telomeres could not be ruled out.
- Research Organization:
- Oak Ridge National Laboratory (ORNL); Mouse Genetics Research Facility
- Sponsoring Organization:
- ORNL LDRD Director's R&D; SC USDOE - Office of Science (SC)
- DOE Contract Number:
- AC05-00OR22725
- OSTI ID:
- 978252
- Journal Information:
- Cell Cycle, Journal Name: Cell Cycle Journal Issue: 10 Vol. 4; ISSN 1538-4101
- Country of Publication:
- United States
- Language:
- English
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