Community Proteomics of a Natural Microbial Biofilm

Journal Article · Wed Jun 01 04:00:00 EDT 2005 · Science

DOI:https://doi.org/10.1126/science. 1109070· OSTI ID:978041

Ram, Rachna J ^[1]; Verberkmoes, Nathan C ^[2]; Thelen, Michael P ^[1]; Tyson, Gene W ^[1]; Baker, Brett J ^[1]; Shah, Manesh B ^[2]; BlakeII, Robert C ^[3]; Banfield, Jillian F ^[1]

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1. University of California, Berkeley
2. ORNL
3. Xavier University

Using genomic and mass spectrometry-based proteomic methods, we evaluated gene expression, identified key activities, and examined partitioning of metabolic functions in a natural acid mine drainage (AMD) microbial biofilm community. We detected 2033 proteins from the five most abundant species in the biofilm, including 48% of the predicted proteins from the dominant biofilm organism, Leptospirillum group II. Proteins involved in protein refolding and response to oxidative stress appeared to be highly expressed, which suggests that damage to biomolecules is a key challenge for survival. We validated and estimated the relative abundance and cellular localization of 357 unique and 215 conserved novel proteins and determined that one abundant novel protein is a cytochrome central to iron oxidation and AMD formation.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: SC USDOE - Office of Science (SC)
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 978041
• Journal Information: Science, Journal Name: Science Journal Issue: 0 Vol. 308; ISSN 0193-4511; ISSN SCEHDK
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS
ABUNDANCE
ACID MINE DRAINAGE
CYTOCHROMES
GENES
IRON
OXIDATION
PROTEINS