The distinct proteome of placental malaria parasites.
Malaria proteins expressed on the surface of Plasmodium falciparum infected erythrocytes (IE) mediate adhesion and are targeted by protective immune responses. During pregnancy, IE sequester in the placenta. Placental IE bind to the molecule chondroitin sulfate A (CSA) and preferentially transcribe the gene that encodes VAR2CSA, a member of the PfEMP1 variant surface antigen family. Over successive pregnancies women develop specific immunity to CSA-binding IE and antibodies to VAR2CSA. We used tandem mass spectrometry together with accurate mass and time tag technology to study IE membrane fractions of placental parasites. VAR2CSA peptides were detected in placental IE and in IE from children, but the MC variant of VAR2CSA was specifically associated with placental IE. We identified six conserved hypothetical proteins with putative TM or signal peptides that were exclusively expressed by the placental IE, and 11 such proteins that were significantly more abundant in placental IE. One of these hypothetical proteins, PFI1785w, is a 42kDa molecule detected by Western blot in parasites infecting pregnant women but not those infecting children.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 977340
- Report Number(s):
- PNNL-SA-61372; 26194; KP1704020
- Journal Information:
- Molecular and Biochemical Parasitology, 155(1):57-65, Journal Name: Molecular and Biochemical Parasitology, 155(1):57-65 Journal Issue: 1 Vol. 155
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
99 GENERAL AND MISCELLANEOUS
ADHESION
ANTIBODIES
ANTIGENS
CHILDREN
CHONDROITIN
Comparative proteomic
ERYTHROCYTES
Environmental Molecular Sciences Laboratory
GENES
IMMUNITY
MALARIA
MASS SPECTROSCOPY
MEMBRANES
Membrane-associated proteins
PARASITES
PEPTIDES
PLACENTA
PLASMODIUM
PREGNANCY
PROTEINS
Placental malaria
Plasmodium falciparum
SULFATES