Involvement of a Membrane-Bound Class III Adenylate Cyclase in Regulation of Anaerobic Respiration in Shewanella oneidensis MR-1

Journal Article · Wed Jul 01 04:00:00 EDT 2009 · Journal of Bacteriology, 191(13):4298–4306

DOI:https://doi.org/10.1128/JB.01829-08· OSTI ID:974475

Charania, M; Brockman, K; Zhang, Yang; Banerjee, A; Pinchuk, Grigoriy; Fredrickson, Jim K; Beliaev, Alex S; Saffarini, Daad

Unlike other bacteria that use FNR to regulate anaerobic respiration, S. oneidensis MR-1 uses the cAMP receptor protein, CRP, for this purpose. Three putative genes, cyaA, cyaB, and cyaC, predicted to encode class I, class IV, and class III adenylate cyclases respectively, have been identified in the genome sequence of this bacterium. Functional validation through complementation of an E. coli cya mutant confirmed that these genes encode proteins with adenylate cyclase activities. Chromosomal deletion of either cyaA or cyaB did not affect anaerobic respiration with fumarate, DMSO, or Fe(III), whereas the deletion of cyaC caused deficiencies in respiration with DMSO and Fe(III), and to a lesser extent with fumarate. A phenotype similar to that of a crp mutant, which lacks the ability to grow anaerobically with DMSO, fumarate, and Fe(III), was obtained when both cyaA and cyaC were deleted. Microarray analysis of gene expression in the crp and the cyaC mutants revealed the involvement of both genes in the regulation of key respiratory pathways such as DMSO, fumarate, and Fe(III) reduction. Additionally, several genes associated with plasmid replication, flagella biosynthesis, and electron transport, were differentially expressed in the cyaC mutant, but not in the crp mutant. Our results indicated that CyaC plays a major role in regulating anaerobic respiration, and may contribute to additional signaling pathways independent of CRP.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 974475
• Report Number(s): PNNL-SA-52138; KP1501021
• Journal Information: Journal of Bacteriology, 191(13):4298–4306, Journal Name: Journal of Bacteriology, 191(13):4298–4306
• Country of Publication: United States
• Language: English

Related Subjects

Shewanella oneidensis
adenylate cyclase
anaerobic respiration
cAMP receptor protein