Proteome of Salmonella enterica serotype Tyhimurium Grown in Low Mg2+/pH Medium

Journal Article · Fri Sep 04 04:00:00 EDT 2009 · Journal of Proteomics and Bioinformatics, 2(9):388-397

DOI:https://doi.org/10.4172/jpb.1000099· OSTI ID:973990

Shi, Liang; Ansong, Charles; Smallwood, Heather S; Rommereim, Leah M; McDermott, Jason E; Brewer, Heather M; Norbeck, Angela D; Taylor, Ronald C; Gustin, Jean K; Heffron, Fred; Smith, Richard D; Adkins, Joshua N

To determine the impact of a low Mg2+/pH defined growth medium (MgM) on the proteome of Salmonella enterica serotype Typhimurium, we cultured S. Typhimurium cells in the medium under two different conditions termed MgM Shock and MgM Dilution and then comparatively analyzed the bacterial cells harvested from these conditions by a global proteomic approach. Proteomic results showed that MgM Shock and MgM Dilution differentially affected the S. Typhimurium proteome. MgM Shock induced a group of proteins whose induction usually occurred at low O2 level, while MgM Dilution induced those related to the type III secretion system (T3SS) of Salmonella Pathogenicity Island 2 (SPI2) and those involved in thiamine or biotin biosynthesis. The metabolic state of the S. Typhimurium cells grown under MgM Shock condition also differed significantly from that under MgM Dilution condition. Western blot analysis not only confirmed the proteomic results, but also showed that the abundances of SPI2-T3SS proteins SsaQ and SseE and biotin biosynthesis proteins BioB and BioD increased after S. Typhimurium infection of RAW 264.7 macrophages. Deletion of the gene encoding BioB reduced the bacterial ability to replicate inside the macrophages, suggesting a biotin-limited environment encountered by S. Typhimurium within RAW 264.7 macrophages.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 973990
• Report Number(s): PNNL-SA-68770; 400412000
• Journal Information: Journal of Proteomics and Bioinformatics, 2(9):388-397, Journal Name: Journal of Proteomics and Bioinformatics, 2(9):388-397 Journal Issue: 9 Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS
BIOSYNTHESIS
BIOTIN
DILUTION
GENES
INDUCTION
MACROPHAGES
PROTEINS
SALMONELLA
SECRETION
Salmonella enterica serotype Typhimurium
Salmonella-containing vacuole
Salmonella Pathogenicity Island 2
THIAMINE
biotin biosynthesis
low Mg2+/pH defined growth medium
type III secretion system