Proteolysis in Plastids of Arabidopsis Thaliana: Functional Analysis of ClpS1,2,T and their Physical and Genetic Interactions with the ClpPR Protease Core Complex and Clp Chaperones
Chloroplasts are essential organelles required for plant growth and biomass production. They synthesize many essential secondary metabolites (e.g. hormones, isoprenoids, amino acids, etc.) and house the photosynthetic apparatus needed for conversion of light energy and CO2 into chemical energy [in the form of reduced carbohydrates, ATP and NADPH]. Thus chloroplasts are essential for life on earth and essential for production of bioenergy. Formation and maintenance of a functional chloroplast requires an extensive investment in the biogenesis and homeostasis apparatus. Protease and proteolysis play a critical role in these processes, with the Clp gene family being particularly central. Proteolysis of proteins and protein complexes in plastids is poorly understood, and is not only critical for biogenesis, adaptation and maintenance but is also important for plant development. Several years ago, the vanWijk lab identified a large and relatively abundant ClpP/R/S complex, along with ClpC1,C2 and ClpD chaperones and a putative Clp affinity modulator in plastids. So far, no substrate recognition mechanism has been determined for any Clp complex in plants. The purpose of this grant was to initiate functional analysis of three members of the Clp family.
- Research Organization:
- Cornell Univ., Ithaca, NY (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG02-04ER15560
- OSTI ID:
- 971250
- Report Number(s):
- DOE/ER15560-4; TRN: US201009%%244
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AFFINITY
AMINO ACIDS
ARABIDOPSIS
BIOMASS
CARBOHYDRATES
CELL CONSTITUENTS
CHLOROPLASTS
FUNCTIONAL ANALYSIS
FUNCTIONALS
GENES
GENETICS
HOMEOSTASIS
HORMONES
MAINTENANCE
METABOLITES
PLANT GROWTH
PRODUCTION
PROTEINS
PROTEOLYSIS
SUBSTRATES
chloroplasts
photosynthesis
proteolysis