A biological/chemical process for reduced waste and energy consumption, Caprolactam production: Phase 1, Select microorganisms and demonstrate feasibility. Final report

Technical Report · Tue Aug 01 00:00:00 EDT 1995

DOI:https://doi.org/10.2172/96920· OSTI ID:96920

Martin, E J

A novel biological/chemical process for converting cyclohexane into caprolactam was investigated. Microorganisms in a bioreactor would be used to convert cyclohexane into caprolactone followed by chemical synthesis of caprolactam using ammonia. The proposed bioprocess would be more energy efficient and reduce byproducts and wastes that are generated by the current chemical process. We have been successful in isolating from natural soil and water samples two microorganisms that can utilize cyclohexane as a sole source of carbon and energy for growth. These microorganisms were shown to have the correct metabolic intermediates and enzymes to convert cyclohexane into cyclohexanol, cyclohexanone and caprolactone. Genetic techniques to create and select for caprolactone hydrolase negative-mutants are being developed. These blocked-mutants will be used to convert cyclohexane into caprolactone but, because of the block, be unable to metabolize the caprolactone further and excrete it as a final end product.

• Research Organization: Allied-Signal, Inc., Des Plaines, IL (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: FC04-93AL94462
• OSTI ID: 96920
• Report Number(s): DOE/AL/94462--1; ON: DE95016701
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION
55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOSYNTHESIS
CHEMICAL FEEDSTOCKS
COMPARATIVE EVALUATIONS
CYCLOHEXANE
ENZYMATIC HYDROLYSIS
GENE MUTATIONS
HYDROXYLASES
LACTONES
MICROORGANISMS
OXIDOREDUCTASES
PROGRESS REPORT
PROTEIN ENGINEERING