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Use of prolines for improving growth and other properties of plants and algae

Patent ·
OSTI ID:968993

Increasing the concentration of prolines, such as 2-hydroxy-5-oxoproline, in the foliar portions of plants has been shown to cause an increase in carbon dioxide fixation, growth rate, dry weight, nutritional value (amino acids), nodulation and nitrogen fixation, photosynthetically derived chemical energy, and resistance to insect pests over the same properties for wild type plants. This can be accomplished in four ways: (1) the application of a solution of the proline directly to the foliar portions of the plant by spraying these portions; (2) applying a solution of the proline to the plant roots; (3) genetically engineering the plant and screening to produce lines that over-express glutamine synthetase in the leaves which gives rise to increased concentration of the metabolite, 2-hydroxy-5-oxoproline (this proline is also known as 2-oxoglutaramate); and (4) impairing the glutamine synthetase activity in the plant roots which causes increased glutamine synthetase activity in the leaves which gives rise to increased concentration of 2-hydroxy-5-oxoproline. Prolines have also been found to induce similar effects in algae.

Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM
Sponsoring Organization:
United States Department of Energy
DOE Contract Number:
W-7405-ENG-36
Assignee:
The Regents of the University of California (Los Alamos, NM)
Patent Number(s):
6,831,040
Application Number:
09/493,039
OSTI ID:
968993
Country of Publication:
United States
Language:
English

References (7)

Overexpression of a soybean gene encoding cytosolic glutamine synthetase in shoots of transgenic Lotus corniculatus L. plants triggers changes in ammonium assimilation and plant development journal April 1997
Mass cultivation of algae in pure heavy water journal September 1962
Does root glutamine synthetase control plant biomass production in Lotus japonicus L.? journal October 1999
Modulation of glutamine synthetase gene expression in tobacco by the introduction of an alfalfa glutamine synthetase gene in sense and antisense orientation: molecular and biochemical analysis journal January 1993
Oats Tolerant of Pseudomonas syringae pv. tabaci Contain Tabtoxinine-β-Lactam-Insensitive Leaf Glutamine Synthetases journal October 1988
Overproduction of alfalfa glutamine synthetase in transgenic tobacco plants journal June 1989
[130] Glutamine synthetase Escherichia coli book January 1970

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