The Infrared Spectra of Bacillus Bacteria Part I: Vegetative Bacillus versus Sporulated Cells and the Contributions of Phospholipids to Vegetative Infrared Spectra
This paper highlights the distinctions between the IR absorption spectra of vegetative versus sporulated Bacillus bacteria. It is observed that there are signatures clearly associated with either the sporulated or the vegetative state, and that vegetative cell (debris) can contribute to the spore spectra. A distinct feature at ~1739 cm-1 appears to be unique to vegetative cell spectra, and can also be used as an indicator of vegetative cells or cell debris in the spore spectra. The data indicate the band is caused by a phospholipid carbonyl bond and are consistent with, but do not prove, it to be either phosphatidyl ethanolamine (PE) or phosphatidyl glycerol (PG), the two major classes of phospholipids found in vegetative cells of Bacillus species. A companion paper discusses features associated with the sporulated state.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 963576
- Report Number(s):
- PNNL-SA-64040; NN2001000
- Journal Information:
- Applied Spectroscopy, 63(8):899-907, Journal Name: Applied Spectroscopy, 63(8):899-907 Journal Issue: 8 Vol. 63; ISSN 0003-7028; ISSN APSPA4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Infrared signatures of Bacillus bacteria: Clear IR distinctions between sporulated and vegetative cells with chemical assignments
The Infrared Spectra of Bacillus Bacteria Part II: Sporulated Bacillus-the Effect of Vegetative Cells and Contributions of Calcium Dipicolinate Trihydrate, CaDP•3H2O