Versatile P(acman) BAC Libraries for Transgenesis Studies in Drosophila melanogaster

Journal Article · Tue Apr 21 04:00:00 EDT 2009 · Nature Methods

DOI:https://doi.org/10.1038/nmeth.1331· OSTI ID:962653

Venken, Koen J.T.; Carlson, Joseph W; Schulze, Karen L; Pan, Hongling; He, Yuchun; Spokony, Rebecca; Wan, Kenneth H; Koriabine, Maxim; de Jong, Pieter J; White, Kevin P; Bellen, Hugo J; Hoskins, Roger A

We constructed Drosophila melanogaster BAC libraries with 21-kb and 83-kb inserts in the P(acman) system. Clones representing 12-fold coverage and encompassing more than 95percent of annotated genes were mapped onto the reference genome. These clones can be integrated into predetermined attP sites in the genome using Phi C31 integrase to rescue mutations. They can be modified through recombineering, for example to incorporate protein tags and assess expression patterns.

• Research Organization: Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
• Sponsoring Organization: Life Sciences Division
• DOE Contract Number: AC02-05CH11231
• OSTI ID: 962653
• Report Number(s): LBNL-1979E
• Journal Information: Nature Methods, Journal Name: Nature Methods Journal Issue: 6 Vol. 6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DROSOPHILA
GENES
MUTATIONS
PROTEINS