Mapping protein-protein interactions with phage-displayed combinatorial peptide libraries.
Journal Article
·
· Curr. Protocols Cell Biol.
This unit describes the process and analysis of affinity selecting bacteriophage M13 from libraries displaying combinatorial peptides fused to either a minor or major capsid protein. Direct affinity selection uses target protein bound to a microtiter plate followed by purification of selected phage by ELISA. Alternatively, there is a bead-based affinity selection method. These methods allow one to readily isolate peptide ligands that bind to a protein target of interest and use the consensus sequence to search proteomic databases for putative interacting proteins.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- DE-AC02-06CH11357
- OSTI ID:
- 961087
- Report Number(s):
- ANL/BIO/JA-44514; TRN: US201010%%915
- Journal Information:
- Curr. Protocols Cell Biol., Journal Issue: 2003
- Country of Publication:
- United States
- Language:
- ENGLISH
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