A humanized anti-M2 scFv shows protective in vitro activity against influenza
Journal Article
·
· Protein Engineering Design and Selection
OSTI ID:960849
- Los Alamos National Laboratory
M2 is one of the most conserved influenza proteins, and has been widely prospected as a potential universal vaccine target, with protection predominantly mediated by antibodies. In this paper we describe the creation of a humanized single chain Fv from 14C2, a potent monoclonal antibody against M2. We show that the humanized scFv demonstrates similar activity to the parental mAb: it is able to recognize M2 in its native context on cell surfaces and is able to show protective in vitro activity against influenza, and so represents a potential lead antibody candidate for universal prophylactic or therapeutic intervention in influenza.
- Research Organization:
- Los Alamos National Laboratory (LANL)
- Sponsoring Organization:
- DOE
- DOE Contract Number:
- AC52-06NA25396
- OSTI ID:
- 960849
- Report Number(s):
- LA-UR-08-07627; LA-UR-08-7627
- Journal Information:
- Protein Engineering Design and Selection, Journal Name: Protein Engineering Design and Selection
- Country of Publication:
- United States
- Language:
- English
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