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Spore Coat Architecture of Clostridium novyi-NT spores

Journal Article · · Journal of Bacteriology
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Spores of the anaerobic bacterium Clostridium novyi-NT are able to germinate in and destroy hypoxic regions of tumors in experimental animals. Future progress in this area will benefit from a better understanding of the germination and outgrowth processes that are essential for the tumorilytic properties of these spores. Towards this end, we have used both transmission electron microscopy and atomic force microscopy to determine the structure of dormant as well as germinating spores. We found that the spores are surrounded by an amorphous layer intertwined with honeycomb parasporal layers. Moreover, the spore coat layers had apparently self-assembled and this assembly was likely to be governed by crystal growth principles. During germination and outgrowth, the honeycomb layers as well as the underlying spore coat and undercoat layers sequentially dissolved until the vegetative cell was released. In addition to their implications for understanding the biology of C. novyi-NT, these studies document the presence of proteinaceous growth spirals in a biological organism.
Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA
Sponsoring Organization:
USDOE
DOE Contract Number:
W-7405-ENG-48
OSTI ID:
957610
Report Number(s):
UCRL-JRNL-230805
Journal Information:
Journal of Bacteriology, Journal Name: Journal of Bacteriology Journal Issue: 17 Vol. 189; ISSN JOBAAY; ISSN 0021-9193
Country of Publication:
United States
Language:
English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARCHITECTURE
ATOMIC FORCE MICROSCOPY
BIOLOGY
CLOSTRIDIUM
CRYSTAL GROWTH
GERMINATION
NEOPLASMS
SPORES
TRANSMISSION ELECTRON MICROSCOPY