Imaging and differentiation of mouse embryo tissues by ToF-SIMS
Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) equipped with a gold ion gun was used to image mouse embryos and differentiate tissue types (brain, spinal cord, skull, rib, heart and liver). Embryos were paraffin-embedded and then de-paraffinized. The robustness and repeatability of the method was determined by analyzing nine tissue slices from three different embryos over a period of several weeks. Using Principal Component Analysis (PCA) to reduce the spectral data generated by ToF-SIMS, histopathologically identified tissue types of the mouse embryos can be differentiated based on the characteristic differences in their mass spectra. These results demonstrate the ability of ToF-SIMS to determine subtle chemical differences even in fixed histological specimens.
- Research Organization:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 957174
- Report Number(s):
- UCRL-JRNL-222584
- Journal Information:
- International Journal of Mass Spectrometry, vol. 260, N/A, February 1, 2007, pp. 137, Journal Name: International Journal of Mass Spectrometry, vol. 260, N/A, February 1, 2007, pp. 137 Vol. 260
- Country of Publication:
- United States
- Language:
- English
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