Experimental Approaches for Solution X-Ray Scattering And Fiber Diffraction
X-ray scattering and diffraction from non-crystalline systems have gained renewed interest in recent years, as focus shifts from the structural chemistry information gained by high-resolution studies to the context of structural physiology at larger length scales. Such techniques permit the study of isolated macromolecules as well as highly organized macromolecular assemblies as a whole under near-physiological conditions. Time-resolved approaches, made possible by advanced synchrotron instrumentation, add a crucial dimension to many of these investigations. This article reviews experimental approaches in non-crystalline X-ray scattering and diffraction that may be used to illuminate important scientific questions such as protein/nucleic acid folding and structure-function relationships in large macromolecular assemblies.
- Research Organization:
- SLAC National Accelerator Lab., Menlo Park, CA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC02-76SF00515
- OSTI ID:
- 953590
- Report Number(s):
- SLAC-REPRINT-2009-293; TRN: US1000850
- Journal Information:
- Curr. Opin. Struct. Biol. 18:601,2008, Vol. 18, Issue 5; ISSN 0959-440X
- Country of Publication:
- United States
- Language:
- English
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